Key Laboratory of Weed Biology and Management, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No.2 Yuanmingyuanxilu, Beijing 100193, China.
Key Laboratory of Weed Biology and Management, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No.2 Yuanmingyuanxilu, Beijing 100193, China.
Pestic Biochem Physiol. 2021 Jun;175:104848. doi: 10.1016/j.pestbp.2021.104848. Epub 2021 Apr 2.
Tausch's goatgrass (Aegilops tauschii Coss.), is a major weed species, infesting wheat (Triticum aestivum) fields in China. 2,4-D isooctyl ester is widely used for broadleaf weed control and selected as a tool to study the differences between, A. tauschii and T. aestivum. In this study, we measured the growth responses of these species to 2,4-D isooctyl ester and found that T. aestivum was more sensitive to the herbicide than A. tauschii. To clarify the reasons for this difference, we measured the leaf-mediated deposition, absorption and metabolism of 2,4-D isooctyl ester and the expression of auxin receptor transport inhibitor response (TIR1) gene in T. aestivum and A. tauschii. The results indicated that the deposition of 2,4-D isooctyl ester droplets may be lower on A. tauschii than on T. aestivum, because of the increased contact angle and greater density of trichomes on the leaves of the former. A distinct increase in 2,4-D isooctyl ester uptake was detected in T. aestivum during the entire experimental period, and the rate was 2.2-fold greater than that in A. tauschii at 6 h after treatment. Compared with A. tauschii, T. aestivum exhibited a greater accumulation of primary metabolite 2,4-D in plants, which may be responsible for the different responses of the two species. Additionally, the absolute expression level of TIR1 was clearly greater in T. aestivum than that in A. tauschii. These data will be helpful to further understand the differences between T. aestivum and A. tauschii, which may provide a unique perspective for the development and identification of new target compounds that are effective against this weed species.
托什山羊草（Aegilops tauschii Coss.）是一种主要的杂草物种，在中国的小麦（Triticum aestivum）田中大量滋生。2,4-D 异辛酯被广泛用于阔叶杂草防治，并被选为研究 A. tauschii 和 T. aestivum 之间差异的工具。在这项研究中，我们测量了这些物种对 2,4-D 异辛酯的生长反应，发现 T. aestivum 比 A. tauschii 对除草剂更敏感。为了阐明这种差异的原因，我们测量了 2,4-D 异辛酯在 T. aestivum 和 A. tauschii 叶片中的沉积、吸收和代谢以及生长素受体运输抑制剂反应（TIR1）基因的表达。结果表明，由于 A. tauschii 叶片上的触角增大和毛密度增加，2,4-D 异辛酯液滴在 A. tauschii 上的沉积可能低于 T. aestivum。在整个实验期间，T. aestivum 中检测到 2,4-D 异辛酯吸收的明显增加，处理后 6 小时的吸收速率比 A. tauschii 高 2.2 倍。与 A. tauschii 相比，T. aestivum 植物中初级代谢物 2,4-D 的积累量更大，这可能是两种物种反应不同的原因。此外，TIR1 的绝对表达水平在 T. aestivum 中明显高于 A. tauschii。这些数据将有助于进一步了解 T. aestivum 和 A. tauschii 之间的差异，这可能为开发和鉴定针对这种杂草物种的新靶标化合物提供独特的视角。
