School of Pharmaceutical Science and Technology, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, Tianjin, 300072, China.
Anal Sci. 2021 Nov 10;37(11):1583-1587. doi: 10.2116/analsci.21P115. Epub 2021 May 14.
The development of simple and sensitive detection methods for tetracyclines (TCs) is crucial for their routine detection. The present study developed a colorimetric method for the detection of TCs based on the in-situ generation of AuNPs, which were subsequently coupled with a gold staining reaction. Briefly, TCs containing phenolic groups reduce HAuCl to form gold nanoparticles (AuNPs) as gold seeds. In the gold staining process, the gold seeds catalyze the reduction of HAuCl by NHOH to form gold atoms that deposit on the surface of AuNPs, resulting in the enlargement of AuNPs. Sensitive detection of TCs was achieved by employing the gold staining technique. As low as 14, 18.9, and 1.98 nM of oxytetracycline (OTC), tetracycline (TC), and doxycycline (DC), respectively, could be sensitively detected. The proposed method also exhibited good repeatability and specificity, and then was applied to the determination of OTC in milk samples.
开发简单、灵敏的四环素（TCs）检测方法对于其常规检测至关重要。本研究基于金纳米粒子（AuNPs）的原位生成，开发了一种用于检测 TCs 的比色法，随后与金染色反应相结合。简要地说，含有酚基团的 TCs 将 HAuCl 还原形成金纳米粒子（AuNPs）作为金种子。在金染色过程中，金种子催化 NHOH 将 HAuCl 还原为沉积在 AuNPs 表面的金原子，导致 AuNPs 增大。通过采用金染色技术实现了对 TCs 的灵敏检测。分别可以灵敏检测低至 14、18.9 和 1.98 nM 的土霉素（OTC）、四环素（TC）和强力霉素（DC）。该方法还表现出良好的重现性和特异性，然后应用于牛奶样品中 OTC 的测定。
