• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

在咪喹莫特诱导的银屑病样小鼠模型中使用桃丹颗粒减轻银屑病性皮炎机制的转录组学分析

Transcriptomic Analysis of the Mechanisms for Alleviating Psoriatic Dermatitis Using Taodan Granules in an Imiquimod-Induced Psoriasis-like Mouse Model.

作者信息

Kuai Le, Luo Ying, Qu Keshen, Ru Yi, Luo Yue, Ding Xiaojie, Xing Meng, Liu Liu, Sun Xiaoying, Li Xin, Li Bin

机构信息

Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Institute of Dermatology, Shanghai Academy of Traditional Chinese Medicine, Shanghai, China.

出版信息

Front Pharmacol. 2021 Apr 14;12:632414. doi: 10.3389/fphar.2021.632414. eCollection 2021.

DOI:10.3389/fphar.2021.632414
PMID:33995034
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8114823/
Abstract

Taodan granules (TDGs) are clinically efficacious for treating psoriasis, buttheir specific mechanisms of action are unclear. In this study, we determined the concentrations of tanshinone IIA and curcumol using high-performance liquid chromatography (HPLC) to establish quality control parameters for assessing the mechanism of TDGs in treating psoriasis. Thereafter, a mouse model of psoriasis was treated with TDGs. TDGs attenuated imiquimod-induced typical erythema, scales, and thickening of the back and ear lesions in the psoriatic mouse model. Furthermore, PCNA and Ki67-positive cells were reduced in the of psoriatic lesions following TDG treatment. Finally, the sequencing results were verified using a multitude of methods, and the mechanism of action of TDGs against psoriasis was found to be via the upregulation of metabolic signaling pathways such as the Gly-Ser-Thr axis, the downregulation of immune and inflammatory pathways, and the decrease in Rac2 and Arhgdib concentrations. Overall, this study clarified the mechanism of TDG treatment for psoriasis and provided evidence for its clinical application.

摘要

桃丹颗粒（TDGs）在临床上对治疗银屑病有效，但其具体作用机制尚不清楚。在本研究中，我们使用高效液相色谱法（HPLC）测定丹参酮IIA和莪术醇的浓度，以建立评估TDGs治疗银屑病机制的质量控制参数。此后，用TDGs治疗银屑病小鼠模型。TDGs减轻了咪喹莫特诱导的银屑病小鼠模型背部和耳部病变的典型红斑、鳞屑和增厚。此外，TDG治疗后银屑病病变处PCNA和Ki67阳性细胞减少。最后，通过多种方法验证测序结果，发现TDGs抗银屑病的作用机制是通过上调Gly-Ser-Thr轴等代谢信号通路、下调免疫和炎症通路以及降低Rac2和Arhgdib浓度。总体而言，本研究阐明了TDG治疗银屑病的机制，并为其临床应用提供了依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64a3/8114823/9f453c90521b/fphar-12-632414-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64a3/8114823/c1a2ed67d23e/fphar-12-632414-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64a3/8114823/47da04e98ed2/fphar-12-632414-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64a3/8114823/58adcbbf82e4/fphar-12-632414-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64a3/8114823/b346d48262c6/fphar-12-632414-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64a3/8114823/9f453c90521b/fphar-12-632414-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64a3/8114823/c1a2ed67d23e/fphar-12-632414-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64a3/8114823/47da04e98ed2/fphar-12-632414-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64a3/8114823/58adcbbf82e4/fphar-12-632414-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64a3/8114823/b346d48262c6/fphar-12-632414-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64a3/8114823/9f453c90521b/fphar-12-632414-g005.jpg

相似文献

1
Transcriptomic Analysis of the Mechanisms for Alleviating Psoriatic Dermatitis Using Taodan Granules in an Imiquimod-Induced Psoriasis-like Mouse Model.在咪喹莫特诱导的银屑病样小鼠模型中使用桃丹颗粒减轻银屑病性皮炎机制的转录组学分析
Front Pharmacol. 2021 Apr 14;12:632414. doi: 10.3389/fphar.2021.632414. eCollection 2021.
2
Gene set enrichment analysis and ingenuity pathway analysis to verify the impact of Wnt signaling in psoriasis treated with Taodan granules.基因集富集分析和 Ingenuity 通路分析，以验证桃丹颗粒治疗银屑病中 Wnt 信号通路的影响。
Am J Transl Res. 2023 Jan 15;15(1):422-434. eCollection 2023.
3
Modular pharmacology-based approach to identify hub genes and kernel pathways of taodan granules treated psoriasis.基于模块化药理学的方法鉴定桃丹颗粒治疗银屑病的关键基因和核心通路。
J Ethnopharmacol. 2021 Nov 15;280:114485. doi: 10.1016/j.jep.2021.114485. Epub 2021 Aug 1.
4
Uncovering the mechanism of Jueyin granules in the treatment of psoriasis using network pharmacology.运用网络药理学揭示绝银颗粒治疗银屑病的作用机制。
J Ethnopharmacol. 2020 Nov 15;262:113214. doi: 10.1016/j.jep.2020.113214. Epub 2020 Jul 29.
5
Banzhilian formula alleviates psoriasis-like lesions the LCN2/MMP-9 axis based on transcriptome analysis.基于转录组分析，半枝莲配方通过LCN2/MMP-9轴减轻银屑病样病变。
Front Pharmacol. 2023 Mar 6;14:1055363. doi: 10.3389/fphar.2023.1055363. eCollection 2023.
6
L. Ameliorates Imiquimod-Induced Psoriasis-Like Dermatitis and Inhibits Inflammatory Cytokines Production through TLR7/8-MyD88-NF-κB-NLRP3 Inflammasome Pathway.L. 通过 TLR7/8-MyD88-NF-κB-NLRP3 炎性小体途径改善咪喹莫特诱导的银屑病样皮炎并抑制炎症细胞因子的产生。
Molecules. 2019 Jun 7;24(11):2157. doi: 10.3390/molecules24112157.
7
Reducing Flightless I expression decreases severity of psoriasis in an imiquimod-induced murine model of psoriasiform dermatitis.降低无飞行能力 I 表达可减轻咪喹莫特诱导的银屑病样皮炎小鼠模型中银屑病的严重程度。
Br J Dermatol. 2017 Mar;176(3):705-712. doi: 10.1111/bjd.14842. Epub 2016 Dec 10.
8
Small interfering RNA targeting of keratin 17 reduces inflammation in imiquimod-induced psoriasis-like dermatitis.靶向角蛋白17的小干扰RNA可减轻咪喹莫特诱导的银屑病样皮炎中的炎症。
Chin Med J (Engl). 2020 Nov 20;133(24):2910-2918. doi: 10.1097/CM9.0000000000001197.
9
Anti-psoriasis effect of water-processed rosin in mice.水飞松对小鼠的抗银屑病作用。
J Ethnopharmacol. 2019 Oct 5;242:112073. doi: 10.1016/j.jep.2019.112073. Epub 2019 Jul 6.
10
Rutaecarpine inhibited imiquimod-induced psoriasis-like dermatitis via inhibiting the NF-κB and TLR7 pathways in mice.瑞香素通过抑制 NF-κB 和 TLR7 通路抑制咪喹莫特诱导的小鼠银屑病样皮炎。
Biomed Pharmacother. 2019 Jan;109:1876-1883. doi: 10.1016/j.biopha.2018.10.062. Epub 2018 Nov 26.

引用本文的文献

1
Vildagliptin topical ointment: an effective treatment for imiquimod-induced psoriasis in mice.维格列汀外用软膏：对咪喹莫特诱导的小鼠银屑病有效治疗。
J Mol Histol. 2025 Apr 26;56(3):143. doi: 10.1007/s10735-025-10416-4.
2
CRISPR/Cas9-Mediated Knockout and Overexpression Studies Unveil the Role of PD-L1 in Immune Modulation in a Psoriasis-like Mouse Model.CRISPR/Cas9介导的基因敲除和过表达研究揭示了程序性死亡配体1（PD-L1）在银屑病样小鼠模型免疫调节中的作用。
Inflammation. 2025 Apr 3. doi: 10.1007/s10753-025-02281-w.
3
The alleviative effects of canagliflozin on imiquimod-induced mouse model of psoriasis-like inflammation.

本文引用的文献

1
Dysregulation of Ki-67 Expression in T Cells of Children with Autism Spectrum Disorder.自闭症谱系障碍患儿T细胞中Ki-67表达失调。
Children (Basel). 2021 Feb 6;8(2):116. doi: 10.3390/children8020116.
2
FKBP10 promotes proliferation of glioma cells via activating AKT-CREB-PCNA axis.FKBP10 通过激活 AKT-CREB-PCNA 轴促进神经胶质瘤细胞的增殖。
J Biomed Sci. 2021 Feb 9;28(1):13. doi: 10.1186/s12929-020-00705-3.
3
Hematopoietic Cell Kinase (HCK) Is Essential for NLRP3 Inflammasome Activation and Lipopolysaccharide-Induced Inflammatory Response .
卡格列净对咪喹莫特诱导的银屑病样炎症小鼠模型的缓解作用。
Naunyn Schmiedebergs Arch Pharmacol. 2025 Mar;398(3):2695-2715. doi: 10.1007/s00210-024-03406-y. Epub 2024 Sep 10.
4
Effect and mechanism of longkui yinxiao soup in treating psoriasis in mice.龙葵饮消汤治疗小鼠银屑病的效果及机制
Front Pharmacol. 2023 Mar 13;14:1136604. doi: 10.3389/fphar.2023.1136604. eCollection 2023.
5
Banzhilian formula alleviates psoriasis-like lesions the LCN2/MMP-9 axis based on transcriptome analysis.基于转录组分析，半枝莲配方通过LCN2/MMP-9轴减轻银屑病样病变。
Front Pharmacol. 2023 Mar 6;14:1055363. doi: 10.3389/fphar.2023.1055363. eCollection 2023.
6
Gene set enrichment analysis and ingenuity pathway analysis to verify the impact of Wnt signaling in psoriasis treated with Taodan granules.基因集富集分析和 Ingenuity 通路分析，以验证桃丹颗粒治疗银屑病中 Wnt 信号通路的影响。
Am J Transl Res. 2023 Jan 15;15(1):422-434. eCollection 2023.
7
Mechanism of Huoluo Xiaoling Dan in the Treatment of Psoriasis Based on Network Pharmacology and Molecular Docking.基于网络药理学和分子对接的活络消灵丹治疗银屑病的机制
Evid Based Complement Alternat Med. 2022 Feb 27;2022:7053613. doi: 10.1155/2022/7053613. eCollection 2022.
造血细胞激酶（HCK）对NLRP3炎性小体激活和脂多糖诱导的炎症反应至关重要。
Front Pharmacol. 2020 Sep 15;11:581011. doi: 10.3389/fphar.2020.581011. eCollection 2020.
4
Curcumol Inhibits Lung Adenocarcinoma Growth and Metastasis via Inactivation of PI3K/AKT and Wnt/-Catenin Pathway.姜黄素通过抑制 PI3K/AKT 和 Wnt/-Catenin 通路抑制肺腺癌细胞生长和转移。
Oncol Res. 2021 Sep 7;28(7):685-700. doi: 10.3727/096504020X15917007265498. Epub 2020 Jun 16.
5
Role of keratinocytes and immune cells in the anti-inflammatory effects of Tripterygium wilfordii Hook. f. in a murine model of psoriasis.三萜生物碱在银屑病小鼠模型中抗炎作用的角质形成细胞和免疫细胞的作用。
Phytomedicine. 2020 Oct;77:153299. doi: 10.1016/j.phymed.2020.153299. Epub 2020 Aug 13.
6
Pulmonary Adverse Events in Patients Receiving Low-Dose Methotrexate in the Randomized, Double-Blind, Placebo-Controlled Cardiovascular Inflammation Reduction Trial.在随机、双盲、安慰剂对照的心血管炎症减少试验中，接受低剂量甲氨蝶呤治疗的患者的肺部不良事件。
Arthritis Rheumatol. 2020 Dec;72(12):2065-2071. doi: 10.1002/art.41452. Epub 2020 Oct 7.
7
Uncovering the mechanism of Jueyin granules in the treatment of psoriasis using network pharmacology.运用网络药理学揭示绝银颗粒治疗银屑病的作用机制。
J Ethnopharmacol. 2020 Nov 15;262:113214. doi: 10.1016/j.jep.2020.113214. Epub 2020 Jul 29.
8
Curcumol improves cisplatin sensitivity of human gastric cancer cells through inhibiting PI3K/AKT pathway.莪术醇通过抑制PI3K/AKT信号通路提高人胃癌细胞对顺铂的敏感性。
Drug Dev Res. 2020 Dec;81(8):1019-1025. doi: 10.1002/ddr.21719. Epub 2020 Jul 26.
9
Bioinformatics approaches for deciphering the epitranscriptome: Recent progress and emerging topics.用于破译表观转录组的生物信息学方法：最新进展与新兴主题。
Comput Struct Biotechnol J. 2020 Jun 13;18:1587-1604. doi: 10.1016/j.csbj.2020.06.010. eCollection 2020.
10
miR-183-3p suppresses proliferation and migration of keratinocyte in psoriasis by inhibiting GAB1.miR-183-3p 通过抑制 GAB1 抑制银屑病角质形成细胞的增殖和迁移。
Hereditas. 2020 Jul 10;157(1):28. doi: 10.1186/s41065-020-00138-w.