Kim Minchae, Jeong Soongon, Lim Chae Woo, Lee Sung Chul
Department of Life Science (BK21 program), Chung-Ang University, Seoul, South Korea.
Front Plant Sci. 2021 Apr 29;12:646707. doi: 10.3389/fpls.2021.646707. eCollection 2021.
Protein phosphorylation by kinase is an important mechanism for adapting to drought stress conditions. Here, we isolated the () from dehydrated pepper leaf tissue and functionally characterized it. Subcellular localization analysis revealed that the CaDIMK1 protein was localized in the cytoplasm and nucleus. -silenced pepper plants exhibited drought-susceptible phenotypes that were characterized by increased transpiration rates, low leaf temperatures, and decreased stomatal closure. In contrast, -overexpressing (OX) transgenic plants were hypersensitive to abscisic acid (ABA) from germination to adult growth stages. Furthermore, the -OX plants were tolerant to drought stress. The transcript levels of several stress-related genes were high in -OX plants than in wild-type plants. Taken together, our data demonstrate that acts as a positive modulator of drought tolerance and ABA signal transduction in pepper plants.
激酶介导的蛋白质磷酸化是植物适应干旱胁迫条件的重要机制。在此，我们从脱水辣椒叶片组织中分离出（）并对其进行了功能鉴定。亚细胞定位分析表明，CaDIMK1蛋白定位于细胞质和细胞核中。（）基因沉默的辣椒植株表现出干旱敏感表型，其特征为蒸腾速率增加、叶片温度降低和气孔关闭减少。相反，（）过表达（OX）转基因植株从萌发到成年生长阶段对脱落酸（ABA）高度敏感。此外，（）-OX植株对干旱胁迫具有耐受性。在（）-OX植株中，几个与胁迫相关的基因的转录水平高于野生型植株。综上所述，我们的数据表明（）在辣椒植株中作为耐旱性和ABA信号转导的正向调节因子发挥作用。
