Anggita Marla, Nugroho Widagdo Sri, Fibrianto Yuda Heru, Budhi Setyo, Budipitojo Teguh
Postgraduate Student of Veterinary Science Master Program, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
Vet Med Int. 2021 Apr 27;2021:8410175. doi: 10.1155/2021/8410175. eCollection 2021.
Mesenchymal stem cells-conditioned medium (MSC-CM) is the extraction from stem cell medium containing biological substances, including growth factors and cytokines. These substances play roles in the various functions of body regulatory, including bone formation. However, the effect of MSC-CM derived from human umbilical cord injection in femur fracture healing of rats has not been reported previously. This study aims to see the effect of MSC-CM derived from human umbilical cord injection on the callus formation of bone fracture healing in Wistar rats (). A femur fracture in 54 Wistar rats was made by surgery according to the procedure under sterile conditions. After the surgery, rats were divided into 2 groups of 27, respectively. Injection in the control (0.1 mL/kg body weight NaCl) and MSC-CM group (0.1 mL/kg body weight MSC-CM) was performed on weeks 0, 1, 2, 3, 4, 5, 6, 7, and 8 after surgery. Radiographic images and the femur bone samples were taken and collected on days 1, 7, 14, 21, 28, 35, and 60 after surgery. Bone samples were then fixed in Bouin solution. Histologic preparations were done by the paraffin method, by cutting the tissue blocks into 5 m thickness and then staining with Mallory aniline blue staining. The results were analyzed descriptively and quantitatively. The result showed that the soft callus formation occurred rapidly and got wider in the MSC-CM group than that of the control group. The administration of MSC-CM injection postfracture surgery to femur fracture cases in rats was capable to accelerate the callus formation.
间充质干细胞条件培养基（MSC-CM）是从含有生物物质的干细胞培养基中提取的，这些生物物质包括生长因子和细胞因子。这些物质在身体调节的各种功能中发挥作用，包括骨形成。然而，先前尚未报道过源自人脐带注射的MSC-CM对大鼠股骨骨折愈合的影响。本研究旨在观察源自人脐带注射的MSC-CM对Wistar大鼠骨折愈合中骨痂形成的影响。按照无菌条件下的操作程序，通过手术使54只Wistar大鼠发生股骨骨折。手术后，将大鼠分为两组，每组27只。在术后第0、1、2、3、4、5、6、7和8周，对对照组（0.1 mL/kg体重的氯化钠）和MSC-CM组（0.1 mL/kg体重的MSC-CM）进行注射。在术后第1、7、14、21、28、35和60天拍摄并收集X线图像和股骨样本。然后将骨样本固定在Bouin溶液中。通过石蜡法进行组织学制备，将组织块切成5μm厚度，然后用马洛里苯胺蓝染色。对结果进行描述性和定量分析。结果表明，与对照组相比，MSC-CM组的软骨痂形成迅速且更宽。对大鼠股骨骨折病例在骨折手术后注射MSC-CM能够加速骨痂形成。
