Borhanjoo Panid, Singh Navneet, Nath Sridesh, Chowdhury Md Sadakat, Swanson Carl, Kaiser Ryan, Geraghty Patrick, Foronjy Robert F, Chow Lillian
Division of Pulmonary & Critical Care Medicine, Department of Medicine, State University of New York Downstate Medical Center, Brooklyn, NY, USA.
State University of New York Downstate School of Medicine, Brooklyn, NY, USA.
SAGE Open Med. 2021 Apr 28;9:20503121211012521. doi: 10.1177/20503121211012521. eCollection 2021.
Sepsis is one of the leading causes of morbidity and mortality within the healthcare system and remains a diagnostic and therapeutic challenge. A major issue in the diagnosis of sepsis is understanding the pathophysiologic mechanism, which revolves around host immune system activation and dysregulated responses. African Americans are more likely to experience severe sepsis with higher mortality rates compared to the general population. This pilot study characterized multiple inflammatory markers and proteases in plasma of primarily African American and Afro-Caribbean patients with mild sepsis.
Plasma was collected from 16 healthy controls and 15 subjects presenting with sepsis, on admission, and again upon resolution of the signs of sepsis, defined as a resolution of sepsis criteria. Plasma samples were analyzed for cytokines, chemokines, and proteases using multiplex bead assays.
Elevated levels of granulocyte colony-stimulating factor, interleukin-10, interleukin-15, interleukin-1 receptor antagonist, interleukin-8, interferon gamma-induced protein 10, monocyte chemoattractant protein-1, matrix metallopeptidase 12, and cathepsin S were identified in plasma from sepsis patients on admission compared to control subjects. Interleukin-6, interleukin-8, granulocyte colony-stimulating factor, and cathepsin S were reduced in sepsis patients upon clinical resolution of sepsis.
These findings profile the circulating inflammatory cytokines, chemokines, and proteases in African Americans and Afro-Caribbean patients during sepsis. The role of these targets in sepsis needs addressing in this patient population.
脓毒症是医疗系统中发病和死亡的主要原因之一，仍然是诊断和治疗方面的挑战。脓毒症诊断中的一个主要问题是理解病理生理机制，这一机制围绕宿主免疫系统激活和失调反应展开。与普通人群相比，非裔美国人更易发生严重脓毒症且死亡率更高。这项初步研究对主要为非裔美国人和非洲加勒比裔的轻度脓毒症患者血浆中的多种炎症标志物和蛋白酶进行了特征分析。
收集了16名健康对照者和15名脓毒症患者的血浆，在入院时以及脓毒症体征消退时（定义为符合脓毒症标准的消退）再次采集。使用多重微珠分析法分析血浆样本中的细胞因子、趋化因子和蛋白酶。
与对照受试者相比，脓毒症患者入院时血浆中粒细胞集落刺激因子、白细胞介素-10、白细胞介素-15、白细胞介素-1受体拮抗剂、白细胞介素-8、干扰素γ诱导蛋白10、单核细胞趋化蛋白-1、基质金属蛋白酶12和组织蛋白酶S水平升高。脓毒症临床缓解时，脓毒症患者的白细胞介素-6、白细胞介素-8、粒细胞集落刺激因子和组织蛋白酶S水平降低。
这些发现描述了非裔美国人和非洲加勒比裔患者在脓毒症期间循环中的炎症细胞因子、趋化因子和蛋白酶情况。在这一患者群体中，这些靶点在脓毒症中的作用需要进一步研究。
