Jing Jian, Du Zhenhuan, Qin Weiwei
Beijing Key Lab of Genetic Engineering and Biotechnology, College of Life Sciences, Beijing Normal University, Beijing, China.
Front Mol Biosci. 2021 Apr 30;8:634606. doi: 10.3389/fmolb.2021.634606. eCollection 2021.
Thrombotic diseases are usually preceded by a hypercoagulable state in the body. This study aimed to screen potential urinary biomarkers for hypercoagulable state based on proteome analysis. Wistar rats were administered with the hemostatic agent etamsylate to establish hypercoagulable state. Urine samples were collected for proteome analysis. We found 20 proteins with levels more than 1.5-fold in difference between control rats and model rats. We searched human homologs of 20 rat proteins and identified 13 human proteins. Of the 13 human homologous proteins, nine were members of human core urinary proteome. Human homologous proteins of differential proteins were highly expressed in 31 human tissues, especially in the kidneys followed by digestive system and reproductive system. Surprisingly, we did not identify known coagulation factors as differential proteins in the urine of model rats. Hypercoagulable state of the body may not involve direct changes in coagulation factors but causes the changes upstream of the coagulation cascade system. Common differential urinary proteins between different hypercoagulable states suggest some common pathways in the formation of hypercoagulable states and may serve as potential biomarkers for the prevention and treatment of thrombotic diseases.
血栓性疾病通常在身体处于高凝状态之前出现。本研究旨在基于蛋白质组分析筛选高凝状态的潜在尿液生物标志物。给Wistar大鼠施用止血剂酚磺乙胺以建立高凝状态。收集尿液样本进行蛋白质组分析。我们发现20种蛋白质在对照大鼠和模型大鼠之间的水平差异超过1.5倍。我们搜索了20种大鼠蛋白质的人类同源物，并鉴定出13种人类蛋白质。在这13种人类同源蛋白质中，有9种是人类核心尿液蛋白质组的成员。差异蛋白质的人类同源蛋白质在31种人类组织中高表达，尤其是在肾脏中，其次是消化系统和生殖系统。令人惊讶的是，我们在模型大鼠尿液中未将已知凝血因子鉴定为差异蛋白质。身体的高凝状态可能不涉及凝血因子的直接变化，而是导致凝血级联系统上游的变化。不同高凝状态之间常见的差异尿液蛋白质提示了高凝状态形成中的一些共同途径，并且可能作为血栓性疾病预防和治疗的潜在生物标志物。
