Programa de Pós-Graduação em Biologia e Biotecnologia de Microrganismos, Pavilhão Max de Menezes, Universidade Estadual de Santa Cruz, Campus Soane Nazaré de Andrade, Salobrinho, Rodovia Jorge Amado, Km 16, CEP 45662-900 Ilhéus, BA, Brazil.
Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Campus Anísio Teixeira, Rua Hormindo Barros, 58, CEP 45029-094 Vitória da Conquista, BA, Brazil.
Biomed Res Int. 2021 Apr 29;2021:6626249. doi: 10.1155/2021/6626249. eCollection 2021.
In recent years, certain sp. have emerged in health care as an alternative therapy for various diseases. Based on this, this study is aimed at evaluating in vitro the potential probiotics of five lactobacilli strains isolated from pulp of cupuaçu fruit fermentation against and . Our lactobacilli strains were classified as safe for use in humans, and they were tolerant to heat and pH. Our strains were biofilm producers, while hydrophobicity and autoaggregation varied from 13% to 86% and 13% to 25%, respectively. The coaggregation of lactobacilli used in this study with and ranged from 15% to 36% and 32% to 52%, respectively. Antimicrobial activity was present in all tested strains against both pathogens, and the growth of pathogens in coculture was reduced by the presence of our lactobacilli. Also, all tested lactobacilli reduced the pH of the culture, even in incubation with pathogens after 24 hours. The cell-free culture supernatants (CFCS) of all five lactobacilli demonstrated activity against the two pathogens with a halo presence and CFCS characterization assay together with gas chromatography revealed that lactic acid was the most abundant organic acid in the samples (50% to 62%). Our results demonstrated that the organic acid production profile is strain-specific. This study revealed that cupuaçu is a promising source of microorganisms with probiotic properties against genital pathogens. We demonstrated by in vitro tests that our strains have probiotic properties. However, the absence of in vivo tests is a limitation of our work due to the need to evaluate the interaction of our lactobacilli with pathogens in the vaginal mucosa. We believe that these findings may be useful in developing a product containing our lactobacilli and their supernatants in order to support with vaginal health.
近年来，某些种属的益生菌在医疗保健领域作为治疗各种疾病的替代疗法出现。基于此，本研究旨在评估从可可果肉发酵液中分离的五株乳杆菌菌株对和的潜在益生菌作用。我们的乳杆菌菌株被分类为对人类安全使用，且能耐受热和 pH 值。我们的菌株是生物膜产生菌，而疏水性和自聚集性分别为 13%至 86%和 13%至 25%。本研究中使用的乳杆菌与和的共聚作用分别为 15%至 36%和 32%至 52%。所有测试的乳杆菌菌株对两种病原体均具有抗菌活性，且存在我们的乳杆菌时，病原体的生长受到抑制。此外，所有测试的乳杆菌均降低了培养物的 pH 值，即使在与病原体孵育 24 小时后也是如此。所有五株乳杆菌的无细胞培养上清液（CFCS）对两种病原体均具有活性，存在抑菌环且 CFCS 特征分析与气相色谱分析显示，乳酸是样本中最丰富的有机酸（50%至 62%）。我们的结果表明，有机酸的产生模式具有菌株特异性。本研究表明，可可果肉是具有针对生殖道病原体的益生菌特性的微生物的有前途的来源。我们通过体外试验证明了我们的菌株具有益生菌特性。然而，由于需要评估我们的乳杆菌与阴道黏膜中的病原体的相互作用，因此缺乏体内试验是我们工作的局限性。我们认为这些发现可能有助于开发含有我们的乳杆菌及其上清液的产品，以支持阴道健康。
