Clemmons Brooke A, Schneider Liesel G, Melchior Emily A, Lindholm-Perry Amanda K, Hales Kristin E, Wells James E, Freetly Harvey C, Hansen Stephanie L, Drewnoski Mary E, Hartman Sarah J, Myer Phillip R
Department of Animal Science, University of Tennessee, Knoxville, Tennessee 37996, USA.
Present address: Department of Animal and Range Science, New Mexico State, Las Cruces, NM 88003, USA.
Access Microbiol. 2020 Nov 23;3(1):acmi000180. doi: 10.1099/acmi.0.000180. eCollection 2021.
Methane produced by cattle is one of the contributors of anthropogenic greenhouse gas. Methods to lessen methane emissions from cattle have been met with varying success; thus establishing consistent methods for decreasing methane production are imperative. Ferric iron may possibly act to decrease methane by acting as an alternative electron acceptor. The objective of this study was to assess the effect of ferric citrate on the rumen bacterial and archaeal communities and its impact on methane production. In this study, eight steers were used in a repeated Latin square design with 0, 250, 500 or 750 mg Fe/kg DM of ferric iron (as ferric citrate) in four different periods. Each period consisted of a 16 day adaptation period and 5 day sampling period. During each sampling period, methane production was measured, and rumen content was collected for bacterial and archaeal community analyses. Normally distributed data were analysed using a mixed model ANOVA using the GLIMMIX procedure of SAS, and non-normally distributed data were analysed in the same manner following ranking. Ferric citrate did not have any effect on bacterial community composition, methanogenic archaea nor methane production (>0.05). Ferric citrate may not be a viable option to observe a ruminal response for decreases in enteric methane production.
牛产生的甲烷是人为温室气体的贡献者之一。减少牛甲烷排放的方法取得了不同程度的成功；因此，建立一致的减少甲烷产生的方法势在必行。三价铁可能通过作为替代电子受体来减少甲烷。本研究的目的是评估柠檬酸铁对瘤胃细菌和古菌群落的影响及其对甲烷产生的影响。在本研究中，八头阉牛采用重复拉丁方设计，在四个不同时期分别给予0、250、500或750毫克铁/千克干物质的三价铁（以柠檬酸铁形式）。每个时期包括16天的适应期和5天的采样期。在每个采样期，测量甲烷产生量，并收集瘤胃内容物进行细菌和古菌群落分析。对正态分布的数据使用SAS的GLIMMIX过程通过混合模型方差分析进行分析，对非正态分布的数据在排序后以相同方式进行分析。柠檬酸铁对细菌群落组成、产甲烷古菌或甲烷产生均无任何影响（>0.05）。柠檬酸铁可能不是观察瘤胃对肠道甲烷产生减少反应的可行选择。
