Department of Chemistry and Biochemistry, Lipscomb University, One University Park Drive, Nashville, TN 37217 USA.
STAR Protoc. 2021 Apr 21;2(2):100476. doi: 10.1016/j.xpro.2021.100476. eCollection 2021 Jun 18.
Determination of a substrate's surface energy profile is a facile and inexpensive method to indicate the substrate's interfacial thermodynamics with another substance (e.g., microorganisms, biomacromolecules, medical devices, etc). The following protocol details a goniometric method to calculate a substrate's surface energy profile which (1) directly correlates to a substrate's interfacial Gibbs energy (ΔG) and (2) predicts the interfacial interactions with other substances. We also provide a calculation template using advanced mathematics to expedite surface energy profile determination. For complete details on the use and execution of this protocol, please refer to Cavitt et al. (2020).
确定基质的表面能谱是一种简单且廉价的方法，可以指示基质与另一种物质（例如微生物、生物大分子、医疗器械等）的界面热力学。本方案详细介绍了一种测量基质表面能谱的折射计法，该方法（1）与基质的界面吉布斯能（ΔG）直接相关，（2）可预测与其他物质的界面相互作用。我们还提供了一个使用高级数学的计算模板，以加速表面能谱的确定。如需详细了解本方案的使用和执行，请参考 Cavitt 等人（2020 年）的文献。
