Departments of Biology and Cell & Systems Biology, University of Toronto - Mississauga.
Departments of Biology and Cell & Systems Biology, University of Toronto - Mississauga;
J Vis Exp. 2021 Apr 28(170). doi: 10.3791/61273.
The Drosophila optic lobe, comprised of four neuropils: the lamina, medulla, lobula and lobula plate, is an excellent model system for exploring the developmental mechanisms that generate neural diversity and drive circuit assembly. Given its complex three-dimensional organization, analysis of the optic lobe requires that one understand how its adult neuropils and larval progenitors are positioned relative to each other and the central brain. Here, we describe a protocol for the dissection, immunostaining and mounting of larval and adult brains for optic lobe imaging. Special emphasis is placed on the relationship between mounting orientation and the spatial organization of the optic lobe. We describe three mounting strategies in the larva (anterior, posterior and lateral) and three in the adult (anterior, posterior and horizontal), each of which provide an ideal imaging angle for a distinct optic lobe structure.
视网膜、神经节细胞层、小叶和小叶板，是研究产生神经多样性和驱动回路组装的发育机制的极佳模型系统。鉴于其复杂的三维结构，分析复眼需要了解其成年神经叶和幼虫前体相对于彼此以及中央脑的位置。在这里，我们描述了一种用于幼虫和成虫大脑解剖、免疫染色和安装以进行视叶成像的方案。特别强调了安装方向与视叶空间组织之间的关系。我们在幼虫中描述了三种安装策略（前、后和侧）和成虫中的三种（前、后和水平），每种策略都为不同的视叶结构提供了理想的成像角度。
