Department of Microbiology and Oregon State University, Corvallis, Oregon, USA.
Department of Biomedical Sciences, Oregon State University, Corvallis, Oregon, USA.
Zebrafish. 2021 Jun;18(3):207-220. doi: 10.1089/zeb.2020.1955. Epub 2021 May 17.
Intestinal neoplasms and preneoplastic lesions are common in zebrafish research facilities. Previous studies have demonstrated that these neoplasms are caused by a transmissible agent, and two candidate agents have been implicated: a sp. related to and the intestinal parasitic nematode, , and both agents are common in zebrafish facilities. To elucidate the role of these two agents in the occurrence and severity of neoplasia and other intestinal lesions, we conducted two experimental inoculation studies. Exposed fish were examined at various time points over an 8-month period for intestinal histopathologic changes and the burden of and nematodes. Fish exposed to sp. isolated from zebrafish were associated with preneoplastic lesions. Fish exposed to the nematode alone or with the isolate developed severe lesions and neoplasms. Both inflammation and neoplasm scores were associated with an increase in burden. These results support the conclusions that is a strong promoter of intestinal neoplasms in zebrafish and that alone can also cause intestinal lesions and accelerate cancer development in the context of nematode infection.
肠道肿瘤和肿瘤前病变在斑马鱼研究机构中很常见。先前的研究表明，这些肿瘤是由一种可传播的病原体引起的，有两种候选病原体被牵连在内：一种与相关的 sp. 和肠道寄生线虫 ，这两种病原体在斑马鱼设施中很常见。为了阐明这两种病原体在肿瘤发生和严重程度以及其他肠道病变中的作用，我们进行了两项实验性接种研究。在 8 个月的时间里，在不同时间点检查暴露的鱼的肠道组织病理学变化和 的负担以及线虫的负担。暴露于从斑马鱼中分离出的 sp. 的鱼与肿瘤前病变有关。单独暴露于线虫或与分离株一起暴露于线虫的鱼会发展出严重的病变和肿瘤。炎症和肿瘤评分均与 的负担增加有关。这些结果支持以下结论：是斑马鱼肠道肿瘤的强有力促进剂，而线虫感染的情况下，线虫本身也可以引起肠道病变并加速癌症发展。
