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氯胺酮和育亨宾对裸鼹鼠(Heterocephalus glaber)尾部刺痛和热板试验的影响。

The effects of clonidine and yohimbine in the tail flick and hot plate tests in the naked mole rat (Heterocephalus glaber).

机构信息

School of Agriculture and Veterinary Sciences, South Eastern Kenya University, P O Box 170-90200, Kitui, Kenya.

Department of Experimental Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamvej 3B, 2200, Copenhagen, Denmark.

出版信息

BMC Res Notes. 2021 May 17;14(1):191. doi: 10.1186/s13104-021-05607-7.

Abstract

OBJECTIVE

The naked mole rat (NMR) (Heterocephalus glaber) is increasingly considered an important biomedical research model for various conditions like hypoxic brain injury, cancer and nociception. This study was designed to investigate the effects of clonidine and yohimbine, an alpha-2 (α) adrenoceptor agonist and antagonist respectively in the tail flick and hot plate tests.

RESULTS

A significant difference in tail flick latency was noted between saline control and 30 µg/kg clonidine, which was reduced after administration of 30 µg/kg yohimbine. A significant difference in hot plate latency was also noted between saline control and 30 µg/kg clodinine during the periods 30, 45, 60, 75 and 90 min after administration, and between saline control and 10 µg/kg clonidine during 30 min after administration. The hot plate latency by 30 µg/kg clonidine was also reduced by 30 µg/kg yohimbine during 30 min after administration. Since the tail-flick and hot plate tests mediate the effects at spinal and supraspinal levels respectively, the present study indicates the presence and involvement of noradrenergic receptors in thermal antinociception at spinal and supraspinal levels of the NMR, similar to what has been found in other mammals.

摘要

目的

裸鼹鼠(NMR)(Heterocephalus glaber)越来越被认为是一种重要的生物医学研究模型,可用于研究各种疾病,如缺氧性脑损伤、癌症和疼痛感知。本研究旨在研究可乐定和育亨宾对尾巴拍打和热板测试的影响,可乐定和育亨宾分别为α-2(α)肾上腺素受体激动剂和拮抗剂。

结果

尾巴拍打潜伏期在盐水对照组和 30μg/kg 可乐定组之间存在显著差异,给予 30μg/kg 育亨宾后潜伏期降低。在给药后 30、45、60、75 和 90 分钟期间,以及在给药后 30 分钟期间,盐水对照组和 30μg/kg 氯胺酮组之间的热板潜伏期也存在显著差异,而在给药后 30 分钟期间,盐水对照组和 10μg/kg 氯胺酮组之间的热板潜伏期也存在显著差异。给予 30μg/kg 可乐定后 30μg/kg 育亨宾还降低了热板潜伏期。由于尾巴拍打和热板测试分别介导脊髓和脊髓上水平的作用,本研究表明,NMR 脊髓和脊髓上水平的热镇痛作用存在去甲肾上腺素能受体,并参与其中,这与在其他哺乳动物中发现的情况相似。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c591/8130107/a4040ee081a9/13104_2021_5607_Fig1_HTML.jpg

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