靶向 pH 开关铕配合物在活细胞中监测受体内化。
Targeted pH switched europium complexes monitoring receptor internalisation in living cells.
机构信息
Department of Chemistry, Durham University, South Road, Durham DH1 3LE, UK.
Cisbio Bioassays, BP 84175, 30200 Codolet, France.
出版信息
Chem Commun (Camb). 2021 Jun 10;57(47):5814-5817. doi: 10.1039/d1cc01029h.
PMID:34002181
Abstract
We report the design and evaluation of pH responsive luminescent europium(iii) probes that allow conjugation to targeting vectors to monitor receptor internalisation in cells. The approach adopted here can be used to tag proteins selectively and to monitor uptake into more acidic organelles, thereby enhancing the performance of time-resolved internalisation assays that require pH monitoring in real time.
摘要
我们报告了 pH 响应发光铕(III)探针的设计和评估，该探针可与靶向载体连接，以监测细胞中受体的内化。这里采用的方法可用于选择性标记蛋白质并监测其进入酸性细胞器的摄取，从而提高需要实时 pH 监测的时间分辨内化测定的性能。
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