College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, China.
College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, China.
Comp Biochem Physiol C Toxicol Pharmacol. 2021 Oct;248:109081. doi: 10.1016/j.cbpc.2021.109081. Epub 2021 May 15.
As a broad-spectrum with low toxicity, procymidone (PCM), is widely used in agriculture and frequently observed in aquatic system, which may cause some impacts on aquatic organisms. Here, to determine the developmental toxicity of PCM, embryonic and larval zebrafish were exposed to PCM at 0, 1, 10, 100 μg/L in dehydrogenated natural water containing 0.01% acetone for 7 days. The results showed that high concentration of PCM could cause the pericardial edema and increase the heart rates in larval zebrafish, suggesting that PCM had developmental toxicity to zebrafish. We also observed that PCM exposure not only changed the physiological parameters including TBA, GLU and pyruvic acid, but also changed the transcriptional levels of glycolipid metabolism related genes. In addition, after transcriptomics analysis, a total of 1065 differentially expressed genes, including 456 up-regulated genes and 609 down-regulated genes, changed significantly in 100 μg/L PCM treated larval zebrafish. Interestingly, after GO (Gene Ontology) analysis, the different expression genes (DEGs) were mainly enriched to the three different biology processes including GABA-nervous, lipid Metabolism and response to drug. We also observed that the levels of GABA receptor related genes including gabrg2, gabbr1α, gabbr1 and gabra6α were inhibited by PCM exposure. Interestingly, the swimming distance of larval zebrafish had the tendency to decrease after PCM exposure, indicating that the nervous system was affected by PCM. Taken together, the results confirmed that the fungicide PCM could cause developmental toxicity by influencing the lipid metabolism and GABA mediated nervous system and behavior in larval zebrafish. We believed that the results could provide an important data for the influence of PCM on aquatic animals.
作为一种广谱低毒的杀菌剂，菌核净（PCM）被广泛应用于农业领域，经常在水生生态系统中被检出，这可能会对水生生物造成一定的影响。本研究旨在确定 PCM 的发育毒性，将胚胎和幼鱼斑马鱼暴露于脱氢天然水中的 0、1、10、100μg/L PCM 中 7 天，其中天然水含有 0.01%丙酮。结果表明，高浓度的 PCM 可导致幼鱼出现心包水肿和心率增加，表明 PCM 对斑马鱼具有发育毒性。我们还观察到，PCM 暴露不仅改变了 TBA、GLU 和丙酮酸等生理参数，还改变了糖脂代谢相关基因的转录水平。此外，通过转录组学分析，在 100μg/L PCM 处理的幼鱼中，共有 1065 个差异表达基因发生了显著变化，包括 456 个上调基因和 609 个下调基因。有趣的是，GO（基因本体论）分析后，差异表达基因（DEGs）主要富集到 GABA 神经、脂质代谢和药物反应三个不同的生物学过程。我们还观察到，GABA 受体相关基因包括 gabrg2、gabbr1α、gabbr1 和 gabra6α 的水平受到 PCM 暴露的抑制。有趣的是，PCM 暴露后幼鱼的游泳距离有下降的趋势，表明神经系统受到 PCM 的影响。综上所述，研究结果证实，杀菌剂 PCM 通过影响脂质代谢和 GABA 介导的神经系统和幼鱼行为，导致发育毒性。我们相信，这些结果可以为 PCM 对水生动物的影响提供重要数据。
