Attiyat A S, Matkovich C E, Christian G D
Chemistry Department, Yarmouk University, Irbid, Jordan.
Am Ind Hyg Assoc J. 1988 May;49(5):244-7. doi: 10.1080/15298668891379684.
Whole blood sample (2 mL) is dry ashed and taken up in 3 M hydrochloric acid. The volume is adjusted to 2 mL with water, and 3 mL of saturated calcium chloride solution (previously saturated with acetone) is added. Iron is removed first by solvent extraction from the acid solution with 100 ppm dithizone in acetone. The pH of the aqueous phase is adjusted to 8.0 with 0.2 mL of 0.4 M tris buffer, and the manganese is extracted with dithizone in 2 mL acetone and is measured in the acetone phase at 278.8 nm by atomic absorption spectrometry using an air-acetylene flame. The mean manganese value found for 14 samples was 32 +/- 7 ppb. The detection limit is 2 ppb manganese.
取2 mL全血样本进行干灰化处理，然后用3 M盐酸溶解。加水将体积调至2 mL，再加入3 mL饱和氯化钙溶液（事先用丙酮饱和）。先用丙酮中100 ppm双硫腙从酸性溶液中进行溶剂萃取去除铁。用0.2 mL 0.4 M三羟甲基氨基甲烷缓冲液将水相pH调至8.0，然后用双硫腙在2 mL丙酮中萃取锰，并用空气 - 乙炔火焰原子吸收光谱法在278.8 nm处测定丙酮相中的锰。14个样本的锰平均含量为32±7 ppb。检测限为2 ppb锰。
