Liu F Y, Zhang Q, Guo X X, Song X, Zhang C X
Shandong Institute of Parasitic Diseases, Shandong First Medical University & Shandong Academy of Medical Sciences, Jining 272000, China.
Clinical Laboratory, Affiliated Hosptial of Jining Medical University, China.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2021 Apr 16;33(2):189-194. doi: 10.16250/j.32.1374.2020347.
To compare the differentially expressed proteins between cypermethrin-resistant and -sensitive , so as to unravel the mechanism underlying the resistance to cypermethrin in .
A quantitative proteomic analysis was performed among cypermethrin-sensitive and -resistant isolates of using isobaric tags for relative and absolute quantification (iTRAQ) labeling coupled with liquid chromatography with tandem mass spectrometry (LC-MS/MS).
A total of 164 differentially expressed proteins were identified between cypermethrin-sensitive and -resistant isolates of , including 54 up-regulated proteins and 110 down-regulated proteins. A large number of cuticular proteins, larval cuticular proteins, pupal cuticular proteins and cuticular structural constituent proteins, which are associated with cytoskeletal structure and components, were differentially expressed between cypermethrin-sensitive and -resistant isolates of . Thirteen proteins, which were involved in energy production and conversion, translation, ribosomal structure and biogenesis, lipid transport and metabolism, post-translational modification, protein turnover, chaperones, cytoskeleton and intracellular transportation, were validated to be differentially expressed between cypermethrin-sensitive and -resistant isolates of , which may serve as potential markers of cypermethrin resistance.
Multiple insecticide resistance mechanisms contribute to the resistance to cypermethrin in , including cuticular resistance and metabolic resistance, and the cuticular protein genes and cytochrome P450 enzymes may play an important role in the resistance of to cypermethrin.
比较氯氰菊酯抗性和敏感品系之间差异表达的蛋白质，以揭示[具体昆虫名称]对氯氰菊酯产生抗性的潜在机制。
采用相对和绝对定量的等压标签（iTRAQ）标记结合液相色谱-串联质谱（LC-MS/MS）技术，对[具体昆虫名称]的氯氰菊酯敏感和抗性品系进行定量蛋白质组学分析。
在[具体昆虫名称]的氯氰菊酯敏感和抗性品系之间共鉴定出164种差异表达蛋白质，其中上调蛋白质54种，下调蛋白质110种。大量与细胞骨架结构和成分相关的表皮蛋白、幼虫表皮蛋白、蛹表皮蛋白和表皮结构组成蛋白在[具体昆虫名称]的氯氰菊酯敏感和抗性品系之间差异表达。有13种蛋白质参与能量产生与转换、翻译、核糖体结构与生物合成、脂质转运与代谢、翻译后修饰、蛋白质周转、分子伴侣、细胞骨架和细胞内运输，经证实在[具体昆虫名称]的氯氰菊酯敏感和抗性品系之间差异表达，可能作为氯氰菊酯抗性的潜在标志物。
多种杀虫剂抗性机制导致[具体昆虫名称]对氯氰菊酯产生抗性，包括表皮抗性和代谢抗性，表皮蛋白基因和细胞色素P450酶可能在[具体昆虫名称]对氯氰菊酯的抗性中发挥重要作用。
