[Study on the mechanism of resistance to cypermethrin in using proteomics].

OBJECTIVE

To compare the differentially expressed proteins between cypermethrin-resistant and -sensitive , so as to unravel the mechanism underlying the resistance to cypermethrin in .

METHODS

A quantitative proteomic analysis was performed among cypermethrin-sensitive and -resistant isolates of using isobaric tags for relative and absolute quantification (iTRAQ) labeling coupled with liquid chromatography with tandem mass spectrometry (LC-MS/MS).

RESULTS

A total of 164 differentially expressed proteins were identified between cypermethrin-sensitive and -resistant isolates of , including 54 up-regulated proteins and 110 down-regulated proteins. A large number of cuticular proteins, larval cuticular proteins, pupal cuticular proteins and cuticular structural constituent proteins, which are associated with cytoskeletal structure and components, were differentially expressed between cypermethrin-sensitive and -resistant isolates of . Thirteen proteins, which were involved in energy production and conversion, translation, ribosomal structure and biogenesis, lipid transport and metabolism, post-translational modification, protein turnover, chaperones, cytoskeleton and intracellular transportation, were validated to be differentially expressed between cypermethrin-sensitive and -resistant isolates of , which may serve as potential markers of cypermethrin resistance.

CONCLUSIONS

Multiple insecticide resistance mechanisms contribute to the resistance to cypermethrin in , including cuticular resistance and metabolic resistance, and the cuticular protein genes and cytochrome P450 enzymes may play an important role in the resistance of to cypermethrin.