Aba-Seq：基因组 5-羟甲基胞嘧啶的高分辨率酶图谱
Aba-Seq: High-Resolution Enzymatic Mapping of Genomic 5-Hydroxymethylcytosine.
机构信息
New England Biolabs Inc., Ipswich, MA, USA.
出版信息
Methods Mol Biol. 2021;2272:13-27. doi: 10.1007/978-1-0716-1294-1_2.
PMID:34009606
Abstract
Aba-Seq (DNA modification-dependent restriction endonuclease AbaSI coupled with sequencing) provides a cost-effective and non-chemical based method for the high-resolution mapping of genomic 5-hydroxymethylcytosine (5hmC). The high specificity of the AbaSI enzyme allows sensitive detection of 5hmC in the genome. Here, we describe the Aba-Seq technique that was used for the high-resolution mapping of 5hmC in the genome of mouse embryonic stem cells (E14).
摘要
Aba-Seq（与测序相结合的依赖于 DNA 修饰的限制性内切酶 AbaSI）为高分辨率绘制基因组 5-羟甲基胞嘧啶（5hmC）图谱提供了一种经济高效且非基于化学的方法。AbaSI 酶的高特异性允许在基因组中灵敏地检测 5hmC。在这里，我们描述了 Aba-Seq 技术，该技术用于绘制小鼠胚胎干细胞（E14）基因组中 5hmC 的高分辨率图谱。
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