Centre de Biotechnologies végétales et microbiennes, Biodiversité et Environnement, Faculty of Sciences, Mohammed V University in Rabat, 4 Avenue Ibn Battouta, Agdal, Rabat, Morocco.
Geoscience and Environment Team Geology Laboratory, CRMEF RABAT, Rabat, Morocco.
Syst Appl Microbiol. 2021 May;44(3):126207. doi: 10.1016/j.syapm.2021.126207. Epub 2021 Apr 18.
In the Moroccan Middle Atlas, the tailings rich in lead and other metal residues, in the abandoned Zaida mining district, represent a real threat to environment and the neighboring villages' inhabitants' health. In this semi-arid to arid area, phytostabilisation would be the best choice to limit the transfer of heavy metals to populations and groundwater. The aim of this work was to characterize the bacteria that nodulate Retama sphaerocarpa, spontaneous nitrogen fixing shrubby legume, native to the Zaida mining area, with great potential to develop for phytostabilisation. Forty-three bacteria isolated from root nodules of the plant were characterized. Based on REP-PCR and ARDRA, four strains were selected for further molecular analyzes. The 16S rRNA gene sequences analysis revealed that the isolated strains are members of the genus Bradyrhizobium, and the phylogenetic analysis of the housekeeping genes glnII, atpD, gyrB, rpoB, recA and dnaK individual sequences and their concatenation showed that the strains are close to B. algeriense RST89 and B. valentinum LmjM3 with similarity percentages of 89.07% to 95.66% which suggest that the newly isolated strains from this mining site may belong to a potential novel species. The phylogeny of the nodA and nodC genes showed that the strains belong to the symbiovar retamae of the genus Bradyrhizobium. These strains nodulate also R. monosperma, R. dasycarpa and Lupinus luteus.
在摩洛哥中阿特拉斯山脉，废弃的扎伊达矿区富含铅和其他金属残留物的尾矿，对环境和附近村庄居民的健康构成了严重威胁。在这个半干旱到干旱地区，植物稳定化将是限制重金属向人口和地下水转移的最佳选择。本工作的目的是对自发固氮灌木豆科植物 Retama sphaerocarpa 的根瘤中的细菌进行特征分析，该植物原产于扎伊达矿区，具有很大的用于植物稳定化的开发潜力。从植物根瘤中分离出 43 株细菌并进行了鉴定。基于 REP-PCR 和 ARDRA，选择了 4 株细菌进行进一步的分子分析。16S rRNA 基因序列分析表明，分离出的菌株是慢生根瘤菌属的成员，而看家基因 glnII、atpD、gyrB、rpoB、recA 和 dnaK 单个序列及其串联的系统发育分析表明，这些菌株与 B. algeriense RST89 和 B. valentinum LmjM3 具有 89.07%至 95.66%的相似性，这表明该矿区新分离的菌株可能属于一个潜在的新物种。nodA 和 nodC 基因的系统发育分析表明，这些菌株属于慢生根瘤菌属的 symbiovar retamae。这些菌株还可以结瘤 R. monosperma、R. dasycarpa 和 Lupinus luteus。
