Banner C, Hwang J J, Shapiro R A, Wenthold R J, Nakatani Y, Lampel K A, Thomas J W, Huie D, Curthoys N P
Laboratory of Molecular Biology, NINCDS, Bethesda, MD 20892.
Brain Res. 1988 Jun;427(3):247-54. doi: 10.1016/0169-328x(88)90047-2.
A single phage was isolated from a lambda gt11 rat brain cDNA library by screening with antibodies prepared against rat renal glutaminase. Partial proteolysis of the fusion protein produced by a lysogen of the isolated phage generated a series of immunoreactive peptides that co-migrated with those derived from the purified brain glutaminase. The cDNA has a single open reading frame which encodes 326 amino acids that are in frame with beta-galactosidase. A 72-kDa protein, corresponding in size to the precursor of mitochondrial glutaminase, was immunoprecipitated from the translation products of rat renal mRNA that selectively hybridized to the cDNA. A probe made from the glutaminase cDNA detected an mRNA about 6 kb in length. This mRNA was present in rat brain and normal kidney RNA, increased 6-fold in acidotic kidney RNA, but was not detectable in liver RNA.
通过用针对大鼠肾谷氨酰胺酶制备的抗体进行筛选，从λgt11大鼠脑cDNA文库中分离出一个单一噬菌体。对分离出的噬菌体的溶原菌产生的融合蛋白进行部分蛋白酶解，产生了一系列免疫反应性肽，这些肽与纯化的脑谷氨酰胺酶衍生的肽共同迁移。该cDNA有一个单一的开放阅读框，编码326个氨基酸，与β-半乳糖苷酶读框一致。从与该cDNA选择性杂交的大鼠肾mRNA的翻译产物中免疫沉淀出一种72 kDa的蛋白质，其大小与线粒体谷氨酰胺酶的前体相对应。由谷氨酰胺酶cDNA制成的探针检测到一种约6 kb长的mRNA。这种mRNA存在于大鼠脑和正常肾RNA中，在酸中毒肾RNA中增加了6倍，但在肝RNA中未检测到。
