Chen Chenchen, Lv Yanhua
Department of Gynecology, Affiliated Hospital of Jining Medical University Jining 272000, Shandong Province, China.
Am J Transl Res. 2021 Apr 15;13(4):2976-2982. eCollection 2021.
This study aimed to investigate the biological behavior of drug-resistant ovarian cancer cells and changes in the cancer antigen 125 (CA125) and human epididymal protein 4 (HE4) levels after the application of cytokine-induced killer (CIK) intervention.
Drug-resistant ovarian cancer cells (namely SKVCR) were treated with CIK at different concentrations to observe the changes in the cell survival and cell morphology and the CA125, HE4, cytokine transforming growth factor-α (TGF-α), and tumor necrosis factor-α (TNF-α) levels in the cell lines before and after intervention.
With an increase in the CIK concentration, the survival rate of the SKVCR cell lines showed a decreasing trend. Under a constant CIK concentration, the survival rate of the SKVCR cell lines gradually decreased over time but become stable at 72 h. Before the CIK intervention, the SKVCR cells were full and rounded in shape, but after the CIK intervention, there was remarkable cell shrinkage and an increase in apoptotic cells. Compared with before the CIK intervention, the CA125 and HE4 levels were significantly decreased, but the TGF-α and TNF-α levels were increased (<0.05).
After the CIK intervention in the drug-resistant ovarian cancer cells, the cell survival rate decreases with an increase in the CIK concentration or an extension of the intervention time, and the cell morphology will be significantly improved, and the CA125, HE4, and other related cytokine levels will also change significantly, suggesting that CIK can kill drug-resistant ovarian cancer cells.
本研究旨在探讨耐药卵巢癌细胞的生物学行为以及细胞因子诱导的杀伤细胞（CIK）干预后癌抗原125（CA125）和人附睾蛋白4（HE4）水平的变化。
用不同浓度的CIK处理耐药卵巢癌细胞（即SKVCR），观察细胞存活和细胞形态的变化以及干预前后细胞系中CA125、HE4、细胞因子转化生长因子-α（TGF-α）和肿瘤坏死因子-α（TNF-α）水平。
随着CIK浓度的增加，SKVCR细胞系的存活率呈下降趋势。在CIK浓度恒定的情况下，SKVCR细胞系的存活率随时间逐渐降低，但在72小时时趋于稳定。CIK干预前，SKVCR细胞饱满且呈圆形，但CIK干预后，细胞明显皱缩，凋亡细胞增加。与CIK干预前相比，CA125和HE4水平显著降低，但TGF-α和TNF-α水平升高（<0.05）。
CIK干预耐药卵巢癌细胞后，细胞存活率随CIK浓度增加或干预时间延长而降低，细胞形态将得到显著改善，CA125、HE4等相关细胞因子水平也会发生显著变化，提示CIK可杀伤耐药卵巢癌细胞。
