Madl J E, Clements J R, Beitz A J, Wenthold R J, Larson A A
Department of Veterinary Biology, University of Minnesota, St. Paul 55108.
Brain Res. 1988 Jun 14;452(1-2):396-402. doi: 10.1016/0006-8993(88)90047-9.
Monoclonal antibodies to glutamate dehydrogenase (GDH) were produced and shown to have high degrees of specificity using immunoblots and ELISA. Immunocytochemical staining of electron microscopic preparations revealed selective intense staining of mitochondria in Bergmann glia, oligodendrocytes and astrocytes in the cerebellum of the rat. Differential intensity of staining among mitochondria within individual glial cells and between glial cells was observed and may provide an anatomical means of detecting differences in glutamate metabolism.
制备了针对谷氨酸脱氢酶（GDH）的单克隆抗体，并通过免疫印迹和酶联免疫吸附测定（ELISA）证明其具有高度特异性。电子显微镜制剂的免疫细胞化学染色显示，大鼠小脑的伯格曼胶质细胞、少突胶质细胞和星形胶质细胞中的线粒体有选择性的强烈染色。观察到单个胶质细胞内线粒体之间以及胶质细胞之间染色强度的差异，这可能提供一种检测谷氨酸代谢差异的解剖学方法。
