水醇提取物通过抑制链脲佐菌素诱导的糖尿病大鼠中结缔组织生长因子（CTGF）和晚期糖基化终产物受体（RAGE）基因表达来改善糖尿病肾病。
hydro alcoholic extract ameliorates diabetic nephropathy by suppressing connective tissue growth factor (CTGF) and receptor for advanced glycation endproducts (RAGE) gene expression in diabetic rats with streptozotocin.
作者信息
Alaee Mona, Amri Jamal, Karami Hadi, Latifi Seyed Amirhossein, Salemi Zahra, Salehi Mehdi
机构信息
Traditional and Complementary Medicine Research Center, Arak University of Medical Sciences, Arak, I.R. Iran.
Department of Clinical Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, I.R. Iran.
出版信息
Horm Mol Biol Clin Investig. 2021 May 20;42(2):167-174. doi: 10.1515/hmbci-2020-0072.
OBJECTIVES
Diabetic nephropathy is one of the major complications of diabetes, the use of medicinal plants is increasing due to fewer side effects. This study was designed to examine antidiabetic effects of () ethanolic extract and evaluate its effects on oxidative stress markers and the expression of connective tissue growth factor (CTGF) and receptor for advanced glycation endproducts (RAGE) genes in the kidney of type 1 diabetic rats.
METHODS
In this study, we randomly divided 24 rats into four groups with six rats in each group as follows: Cnt group: normal control receiving normal saline, Dibt group: diabetic control receiving normal saline daily, Dibt + 250 group: diabetic rats receiving at a dose of 250 mg/kg bw daily, Dibt + 500 group: diabetic rats receiving at a dose of 500 mg/kg bw daily. To induce diabetes, we used 55 mg/kg bw dose of streptozotocin intraperitoneally. The concentration of fasting blood glucose (FBG) and serum urea, creatinine and albumin, SOD, MDA (using spectrophotometric methods) and gene expression of CTGF and RAGE in kidney tissue (using real-time PCR methods) were quantified in the diabetic rats that received for 42 days, and were compared to control rats.
RESULTS
The results showed that in the diabetic group the FBG and serum urea, creatinine and expression of kidney CTGF and RAGE genes and the levels of SOD and MDA significantly increased and serum albumin significantly decreased compared to the Cnt group (p<0.001). Administration of significantly improved the FBG and serum urea, creatinine and albumin compared to Dibt group (p<0.05). It was shown the significantly decrease the kidney expression levels of CTGF and RAGE genes and improve oxidative stress (increased SOD and decreased MDA) in the kidney tissues when compared to Dibt group (p<0.001). Also, it was found that the beneficial effects of the were dose-dependent.
CONCLUSIONS
The results of this study showed that administration of for 42 days has beneficial anti-diabetic and anti-nephropathic effects in diabetic rats and can be used as an adjunct therapy in the treatment of diabetes.
目的
糖尿病肾病是糖尿病的主要并发症之一，由于药用植物副作用较少，其使用正在增加。本研究旨在检测（）乙醇提取物的抗糖尿病作用，并评估其对1型糖尿病大鼠肾脏氧化应激标志物、结缔组织生长因子（CTGF）和晚期糖基化终产物受体（RAGE）基因表达的影响。
方法
在本研究中，我们将24只大鼠随机分为四组，每组6只，分组如下：Cnt组：接受生理盐水的正常对照组；Dibt组：每日接受生理盐水的糖尿病对照组；Dibt + 250组：每日接受剂量为250 mg/kg bw的（提取物）的糖尿病大鼠组；Dibt + 500组：每日接受剂量为500 mg/kg bw的（提取物）的糖尿病大鼠组。为诱导糖尿病，我们腹腔注射55 mg/kg bw剂量的链脲佐菌素。对接受（提取物）42天的糖尿病大鼠，定量检测空腹血糖（FBG）、血清尿素、肌酐和白蛋白浓度、超氧化物歧化酶（SOD）、丙二醛（MDA）（采用分光光度法）以及肾脏组织中CTGF和RAGE的基因表达（采用实时聚合酶链反应法），并与对照大鼠进行比较。
结果
结果显示，与Cnt组相比，糖尿病组的FBG、血清尿素、肌酐以及肾脏CTGF和RAGE基因表达、SOD和MDA水平显著升高，血清白蛋白显著降低（p<0.001）。与Dibt组相比，给予（提取物）显著改善了FBG、血清尿素、肌酐和白蛋白水平（p<0.05）。与Dibt组相比，（提取物）显著降低了肾脏组织中CTGF和RAGE基因的表达水平，并改善了氧化应激（SOD升高，MDA降低）（p<0.001）。此外，还发现（提取物）的有益作用具有剂量依赖性。
结论
本研究结果表明，给予（提取物）42天对糖尿病大鼠具有有益的抗糖尿病和抗肾病作用，可作为糖尿病治疗的辅助疗法。
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