Traditional and Complementary Medicine Research Center, Arak University of Medical Sciences, Arak, Islamic Republic of Iran.
Member of Traditional and Complementary Medicine Research Center, Arak University of Medical Sciences, Arak, Islamic Republic of Iran.
Horm Mol Biol Clin Investig. 2020 Oct 21;41(4):/j/hmbci.ahead-of-print/hmbci-2020-0028/hmbci-2020-0028.xml. doi: 10.1515/hmbci-2020-0028.
The present study was conducted to examine antidiabetic effects of ethanolic extract [] and to investigate its effects on oxidative stress markers and the expression of TLR4, S100A4, Bax and Bcl-2 genes in the kidney of STZ-induced diabetic rats.
Thirty six rats (weight 200-250 g) were randomly divided into diabetes and control groups. Induction of diabetes was performed using STZ (55 mg/kg.bw). Biochemical parameters and oxidative stress markers (SOD and MDA) were measured using spectrophotometry after 60 days of treatment. The expression of TLR4, S100A4, Bax and Bcl-2 were analyzed by real-time PCR. One-way analysis of variance (ANOVA) and Bonferroni post hoc test were used to compare the data.
Diabetes significantly impairs the serum fasting blood glucose (FBG), lipid profile, urea, creatinine and albumin. At the end of treatment with extract, these parameters were close to the normal range. The results showed that the extract significantly decreased the kidney expression of TLR4, S100A4, Bax and increased the expression of Bcl-2 and improved oxidative stress markers (SOD and MDA) in the kidney tissues of treated rats. Also, all of these beneficial effects of the were dose-dependent.
The extract of possesses antidiabetic effects. decreased the expression of TLR4, S100A4, Bax and increased the expression of Bcl-2 and improved oxidative stress. Therefore, this herbal extract can be used as an adjuvant treatment for diabetic complications.
本研究旨在考察[中草药材名称]的抗糖尿病作用，并研究其对链脲佐菌素（STZ）诱导的糖尿病大鼠肾脏氧化应激标志物及 TLR4、S100A4、Bax 和 Bcl-2 基因表达的影响。
36 只大鼠（体重 200-250g）随机分为糖尿病组和对照组。采用 STZ（55mg/kg.bw）诱导糖尿病。治疗 60 天后，采用分光光度法测定生化参数和氧化应激标志物（SOD 和 MDA）。采用实时 PCR 分析 TLR4、S100A4、Bax 和 Bcl-2 的表达。采用单因素方差分析（ANOVA）和 Bonferroni 事后检验比较数据。
糖尿病显著损害血清空腹血糖（FBG）、血脂谱、尿素、肌酐和白蛋白。在用[中草药材名称]提取物治疗结束时，这些参数接近正常范围。结果表明，[中草药材名称]提取物显著降低了肾脏 TLR4、S100A4、Bax 的表达，增加了 Bcl-2 的表达，并改善了治疗大鼠肾脏组织中的氧化应激标志物（SOD 和 MDA）。此外，[中草药材名称]提取物的所有这些有益作用均呈剂量依赖性。
[中草药材名称]提取物具有抗糖尿病作用。它降低了 TLR4、S100A4、Bax 的表达，增加了 Bcl-2 的表达，并改善了氧化应激。因此，这种草药提取物可作为糖尿病并发症的辅助治疗。
