Life Science and Technology College, Guangxi University, Nanning, Guangxi 530004, China.
Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
J Agric Food Chem. 2021 Jun 2;69(21):5917-5925. doi: 10.1021/acs.jafc.1c01557. Epub 2021 May 21.
Polydatin, with better structural stability and biological activities than resveratrol, is mainly extracted from the traditional Chinese medicinal plant . In this study, based on the transcriptome analysis of , we identified the key glycosyltransferase of resveratrol and achieved the biosynthesis of polydatin from glucose by incorporation with the resveratrol biosynthesis module, UDP-glucose supply module, and glycosyltransferase expression module. Through metabolic engineering and fermentation optimization, the production of polydatin reached 545 mg/L, and the dry cell weight was 27.83 mg/g DCW, which was about twice that of extracted from the root (11.404 mg/g DCW). Therefore, it is possible to replace the production mode of polydatin from plant extraction to microbial chassis in the future.
虎杖苷的结构稳定性和生物活性优于白藜芦醇，主要从传统的药用植物中提取。在这项研究中，基于对 的转录组分析，我们鉴定出白藜芦醇的关键糖基转移酶，并通过与白藜芦醇生物合成模块、UDP-葡萄糖供应模块和糖基转移酶表达模块的整合，实现了从葡萄糖到虎杖苷的生物合成。通过代谢工程和发酵优化，虎杖苷的产量达到 545mg/L，干细胞重量达到 27.83mg/gDCW，是从根中提取的（11.404mg/gDCW）的两倍左右。因此，未来有可能将虎杖苷的生产模式从植物提取转变为微生物底盘。
