鸭 CCCH 型锌指抗病毒蛋白 (ZAP) 的分子特征与功能分析。
Molecular characterization and functional analysis of duck CCCH-type zinc finger antiviral protein (ZAP).
机构信息
Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture and Rural Affairs) and Hubei Provincial Key Laboratory of Animal Pathogenic Microbiology, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan, 430064, China; State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture and Rural Affairs) and Hubei Provincial Key Laboratory of Animal Pathogenic Microbiology, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan, 430064, China.
出版信息
Biochem Biophys Res Commun. 2021 Jul 5;561:52-58. doi: 10.1016/j.bbrc.2021.05.019. Epub 2021 May 18.
This is the first study to clone duck CCCH-type zinc finger antiviral protein (duZAP) from Jingjiang duck (Anas platyrhynchos). Full-length duZAP cDNA was 2154 bp and encoded a 717-amino acid polypeptide containing four highly conserved CCCH-type finger motifs, a WWE domain and a poly (ADP-ribose) polymerase (PARP) domain. duZAP was expressed in multiple duck tissues, with the highest mRNA expression in the spleen. Overexpression of duZAP in duck embryo fibroblast cells (DEFs) led to activation of the transcription factors IRF1 and NF-κB, and induction of IFN-β. Analysis of deletion mutants revealed that both the WWE and PARP domains of duZAP were essential for activating the IFN-β promoter. Knockdown of duZAP in DEFs significantly reduced poly (I:C)- and duck Tembusu virus (DTMUV)-induced IFN-β activation. Our findings further the understanding of the role of duZAP in the duck innate immune response.
这是首次从靖江鸭（Anas platyrhynchos）中克隆鸭 CCCH 型锌指抗病毒蛋白（duZAP）的研究。全长 duZAP cDNA 为 2154bp，编码一个包含四个高度保守的 CCCH 型锌指模体、一个 WWE 结构域和一个多聚（ADP-核糖）聚合酶（PARP）结构域的 717 个氨基酸多肽。duZAP 在多种鸭组织中表达，在脾脏中的 mRNA 表达水平最高。在鸭胚胎成纤维细胞（DEFs）中过表达 duZAP 导致转录因子 IRF1 和 NF-κB 的激活，并诱导 IFN-β 的产生。缺失突变体分析表明，duZAP 的 WWE 和 PARP 结构域对于激活 IFN-β 启动子都是必需的。在 DEF 中敲低 duZAP 可显著降低 poly（I:C）和鸭坦布苏病毒（DTMUV）诱导的 IFN-β 激活。我们的研究结果进一步了解了 duZAP 在鸭先天免疫反应中的作用。
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