College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, 150030, China.
College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, 150030, China.
Biochem Biophys Res Commun. 2021 Jul 5;561:88-92. doi: 10.1016/j.bbrc.2021.05.032. Epub 2021 May 18.
Cold-regulated (COR) genes are considered downstream functional genes in the cold-response pathway. However, we identified a plasma membrane-type, AtCor413pm1, as a regulatory gene for the abscisic acid (ABA) response, and found that ABA induced it predominantly in Arabidopsis roots, vasculature, stipules, and guard cells. Differentially expressed genes combined with qPCR analysis revealed the expressions of three ABA-responsive genes (AtDTX50, AtABR1, and AtCIPK20) were significantly altered in the ABA-treated atcor413pm1 mutant, compared to the wild-type. Furthermore, the ABA-induced transient Ca oscillation in the plasma membrane of atcor413pm1 roots was different from that observed in the wild-type. Our results revealed that AtCor413pm1 might play a role in the cross-talk between the ABA and stress response pathways.
冷调节（COR）基因被认为是冷响应途径中的下游功能基因。然而，我们鉴定出一种质膜型 AtCor413pm1 作为脱落酸（ABA）反应的调节基因，并发现 ABA 主要在拟南芥的根、维管束、托叶和保卫细胞中诱导其表达。差异表达基因结合 qPCR 分析表明，与野生型相比，ABA 处理的 atcor413pm1 突变体中三个 ABA 响应基因（AtDTX50、AtABR1 和 AtCIPK20）的表达明显改变。此外，在 atcor413pm1 根质膜中观察到的 ABA 诱导的瞬时 Ca 震荡与野生型观察到的不同。我们的结果表明，AtCor413pm1 可能在 ABA 和应激反应途径之间的串扰中发挥作用。
