School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, Sichuan, China.
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, Sichuan, China.
Res Vet Sci. 2021 Jul;137:194-200. doi: 10.1016/j.rvsc.2021.04.001. Epub 2021 Apr 20.
Fat metabolism is a complex process regulated by a number of factors. Adiponectin receptor 1 (ADIPOR1) gene takes active part in lipid metabolism. Although, there have been some researches indicating that ADIPOR1 could influence the milk fat metabolism through targeting some factors, little is known about the effect of ADIPOR1 on goat milk fat metabolism. To investigate the regulatory role of ADIPOR1 on milk fat metabolism in GMECs, we analysed overexpression in the presence and absence of AdipoRon (50 μM) and examined knockdown using siRNA. Using RT-qPCR, we assessed ADIPOR1 mRNA expressions among different lactation stages in goat mammary gland and the expression of six genes that regulate milk fat metabolism in GMECs.
ADIPOR1 mRNA expression level was higher during the various lactation stages, except dry-off period. Knockdown and overexpression results revealed a significant decrease and increase in mRNA expression of ADIPOR1 and genes considered: SREBF1, ACACA, FASN, SCD, ATGL, and HSL, respectively. Treatment of GMECs with AdipoRon 50 μM resulted in a significant (p < 0.05) increase in the mRNA expression of all measured genes, except SREBF1.
Overall, ADIPOR1 plays a central role in regulating the transcription of several genes involved in milk fat metabolism.
脂肪代谢是一个受多种因素调节的复杂过程。脂联素受体 1（ADIPOR1）基因积极参与脂质代谢。尽管已有一些研究表明 ADIPOR1 可以通过靶向某些因素影响牛奶脂肪代谢，但关于 ADIPOR1 对山羊奶脂肪代谢的影响知之甚少。为了研究 ADIPOR1 在 GMECs 中对奶脂代谢的调节作用，我们在存在和不存在 AdipoRon（50 μM）的情况下分析了过表达，并使用 siRNA 进行了敲低。通过 RT-qPCR，我们评估了不同泌乳阶段山羊乳腺中 ADIPOR1 mRNA 的表达以及 GMECs 中调节奶脂代谢的六个基因的表达。
ADIPOR1 mRNA 表达水平在各个泌乳阶段较高，除了干奶期。敲低和过表达结果显示 ADIPOR1 和以下基因的 mRNA 表达显著降低和增加：SREBF1、ACACA、FASN、SCD、ATGL 和 HSL。GMECs 用 50 μM AdipoRon 处理后，所有测量基因的 mRNA 表达均显著增加（p<0.05），除了 SREBF1。
总之，ADIPOR1 在调节参与乳脂代谢的几个基因的转录中起核心作用。
