Department of Vitamin and Coenzyme Biochemistry, Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine.
Bogomolets National Medical University, Kyiv, Ukraine.
Endocr Regul. 2021 May 21;55(2):61-71. doi: 10.2478/enr-2021-0008.
The present study was designed to assess whether apoptosis-related genes as and could be targets for treatment of diabetes mellitus and whether vitamin D may exert beneficial effects. Vitamin D treatment for 4 weeks, starting after 4 weeks of the diabetes duration. The expression of and genes was estimated on mRNA levels using real time quantitative polymerase chain reaction. After 8 weeks, diabetic rats had weight loss, while blood glucose was increased about 4.9-fold compared to control group. Vitamin D administration to diabetic animals had no effect on these parameters. It was found that total serum alkaline phosphatase activity was significantly elevated in diabetic rats as compared to control animals and was restored by vitamin D. Diabetes was accompanied by reduction of nicotinamidadenindinucleotide, a substrate of poly-ADP-ribosylation, level by 31.7% as compared to control rats, which was not reversed in response to vitamin D treatment. In diabetic hearts, the mRNA expression level of gene was 2.8-fold higher compared to control rats and partially decreased by vitamin D3 treatment. Less significant alterations were observed in diabetic hearts for the mRNA expression level of gene that was 2.0-fold higher compared to control animals and vitamin D normalized it. These results indicate that cardiomyocytes have a tendency to apoptosis. The findings suggest that investigated genes can be targets at the transcriptional level for vitamin D action that may be contributed to the improving metabolic/signaling pathways induced by diabetes mellitus.
本研究旨在评估凋亡相关基因和 是否可作为治疗糖尿病的靶点，以及维生素 D 是否可能发挥有益作用。糖尿病持续 4 周后开始用维生素 D 治疗 4 周。使用实时定量聚合酶链反应估计 和 基因的 mRNA 水平上的表达。8 周后，糖尿病大鼠体重减轻，而血糖与对照组相比增加了约 4.9 倍。维生素 D 给药对这些参数没有影响。研究发现，与对照组相比，糖尿病大鼠的总血清碱性磷酸酶活性显著升高，而维生素 D 可使其恢复正常。糖尿病大鼠的烟酰胺腺嘌呤二核苷酸（多聚 ADP-核糖基化的底物）水平比对照组降低了 31.7%，而这种降低在维生素 D 治疗后并未恢复。与对照组相比，糖尿病大鼠的 基因 mRNA 表达水平高 2.8 倍，而维生素 D3 治疗部分降低了该水平。糖尿病大鼠的 基因 mRNA 表达水平高 2.0 倍，与对照组相比，维生素 D 使其正常化，这种变化不太明显。这些结果表明心肌细胞有凋亡的趋势。研究结果表明，研究中的基因可以作为维生素 D 作用的转录水平靶点，这可能有助于改善糖尿病引起的代谢/信号通路。
