Alrefaie Zienab, Awad Hossam
a Physiology Department, Faculty of Medicine , King Abdulaziz University , Jeddah , Saudi Arabia .
b Physiology Department, Faculty of Medicine , Cairo University , Giza , Egypt , and.
Arch Physiol Biochem. 2015;121(5):206-9. doi: 10.3109/13813455.2015.1107101. Epub 2015 Nov 24.
This study aimed to assess the effect of vitamin D3 administration to diabetic rats on thyroid profile and deiodinase 2 (D2).
Thirty male Wistar rats were included into three groups; control, streptozotocin-induced diabetic and diabetic supplemented with vitamin D3 groups. Ten weeks later, serum levels of free T4, free T3 and TSH were measured. Tissue homogenates from liver, kidney, muscle, femur bone, heart and brain were obtained and assessed for D2 mRNA.
Diabetic rats demonstrated significant increase in free T4 and significant decrease in free T3. These changes were ameliorated by vitamin D3 administration. D2 mRNA was significantly reduced in all tissue homogenates obtained from diabetic rats, while vitamin D3 treatment significantly enhanced D2 in liver and brain homogenates.
Diabetes mellitus inhibited peripheral conversion of T4 into T3 secondary to reduction in D2 expression. Vitamin D3 greatly corrected the alterations in thyroid profile and D2 expression.
本研究旨在评估给予糖尿病大鼠维生素D3对甲状腺功能及脱碘酶2（D2）的影响。
30只雄性Wistar大鼠被分为三组；对照组、链脲佐菌素诱导的糖尿病组和补充维生素D3的糖尿病组。10周后，测量血清游离T4、游离T3和促甲状腺激素（TSH）水平。获取肝脏、肾脏、肌肉、股骨、心脏和大脑的组织匀浆，并评估D2 mRNA水平。
糖尿病大鼠的游离T4显著升高，游离T3显著降低。给予维生素D3可改善这些变化。从糖尿病大鼠获得的所有组织匀浆中，D2 mRNA均显著降低，而维生素D3治疗显著提高了肝脏和脑组织匀浆中的D2水平。
糖尿病继发于D2表达降低，抑制了T4向T3的外周转化。维生素D3极大地纠正了甲状腺功能及D2表达的改变。
