Phillipou G, Seaborn C J, Phillips P J
Endocrine and Diabetes Laboratory, Queen Elizabeth Hospital, Woodville, South Australia.
Clin Chem. 1988 Aug;34(8):1561-4.
The difference in spectral characteristics between 1-deoxy-1-morpholinofructose (DMF) and protein/plasma samples in the fructosamine reaction has been related to the solubility of the diformazan formed by reduction of nitro blue tetrazolium chloride. Addition of the surfactant Triton X-100 (20 g/L) to the reagent buffer not only corrects this anomaly but also enhances the absolute response. Detailed investigation of DMF and dihydroxyacetone as calibration standards for the reaction established a clear preference for the latter. Fundamental differences in reaction kinetics were also noted between the Amadori rearrangement products of glucose formed from morpholine (DMF) or the amino lysine groups of protein (glycated albumin). From the reactivity of dihydroxyacetone, as well as glyceraldehyde, observed in the fructosamine reaction, and the presence of this class of compounds (trioses) in human plasma, we infer that they may also contribute to the differentiation of diabetic and non-diabetic samples.
在果糖胺反应中，1-脱氧-1-吗啉代果糖（DMF）与蛋白质/血浆样品之间的光谱特征差异与氯化硝基四氮唑蓝还原形成的双甲臜的溶解度有关。向试剂缓冲液中添加表面活性剂Triton X-100（20 g/L）不仅可以纠正这种异常情况，还能增强绝对响应。对DMF和二羟基丙酮作为该反应校准标准品的详细研究表明，明显更倾向于使用后者。由吗啉（DMF）形成的葡萄糖的Amadori重排产物或蛋白质的氨基赖氨酸基团（糖化白蛋白）之间在反应动力学上也存在根本差异。从在果糖胺反应中观察到的二羟基丙酮以及甘油醛的反应活性，以及人类血浆中这类化合物（丙糖）的存在，我们推断它们也可能有助于区分糖尿病和非糖尿病样本。
