Laboratório de Biotecnologia Ambiental da Universidade Federal da Integração Latino-Americana - Unila, Av. Tarquínio Joslin Dos Santos, 1000 - Jd Universitário, Foz do Iguaçu, PR, 85870-650, Brazil.
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto Nacional de Medicina Tropical (INMeT) - ANLIS "Dr. Carlos G Malbrán", Almafuerte y Ámbar s/n, 3370, Puerto Iguazú, Misiones, Argentina.
Arch Microbiol. 2021 Sep;203(7):3933-3944. doi: 10.1007/s00203-021-02379-3. Epub 2021 May 21.
The need for more effective drugs for the treatment of infectious diseases as well as for general applications including wound healing and burn surgery, has guided efforts for the discovery of new compounds of medical interest. Microorganisms found in textile industrial waste have the ability to produce a variety of enzymes and/or secondary metabolites including molecules of pharmaceutical interest. The present work investigated the biotechnological potential of filamentous fungi isolated from textile industry wastewater for the production of collagenase and antimicrobial metabolites. From 28 isolates assayed, Sarocladium sp. ITF33 showed specific collagenolytic activity with values of 7.62 and 9.04 U mg for the intracellular and extracellular fractions, respectively. The isolate Penicillium sp. ITF28 showed the best antimicrobial activity, reaching MIC ranging from 1.0 to 0.0625 mg mL against five pathogenic bacteria. Molecular analyzes suggest that the isolate Sarocladium sp. ITF 33 can be considered a species not yet described. The results of the present work encourage studies of characterization and purification of the enzymes and secondary metabolites produced by the isolates found aiming future applications in the medical and pharmaceutical fields.
为了治疗传染病以及包括伤口愈合和烧伤手术在内的一般应用，需要更有效的药物，这指导了人们努力发现具有医学价值的新化合物。在纺织工业废水中发现的微生物具有产生各种酶和/或次级代谢物的能力，包括具有药物价值的分子。本工作研究了从纺织工业废水中分离出的丝状真菌在生产胶原酶和抗菌代谢物方面的生物技术潜力。在所测试的 28 个分离物中，Sarocladium sp. ITF33 表现出特定的胶原酶活性，细胞内和细胞外部分的酶活分别为 7.62 和 9.04 U mg。分离出的青霉 Penicillium sp. ITF28 表现出最好的抗菌活性，对五种致病菌的 MIC 范围为 1.0 至 0.0625 mg mL。分子分析表明，Sarocladium sp. ITF 33 可被视为一种尚未描述的物种。本工作的结果鼓励对分离物产生的酶和次级代谢物进行特性和纯化研究，以期在医学和制药领域的未来应用。
