Igarashi Yuichi, Iida Shiho, Dai Jian, Huo Jia, Cui Xiaoran, Sawashita Jinko, Mori Masayuki, Miyahara Hiroki, Higuchi Keiichi
Department of Aging Biology, Shinshu University Graduate School of Medicine, Matsumoto, Japan.
Department of Neuro-Health Innovation, Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, Matsumoto, Japan.
Biomed Pharmacother. 2021 Aug;140:111714. doi: 10.1016/j.biopha.2021.111714. Epub 2021 May 19.
Glavonoid-rich oil (GRO) derived from ethanol extraction of licorice (Glycyrrhiza glabra Linne) root has been reported to have beneficial effects on health. In this study, we aimed to determine the effect of long-term administration of GRO on metabolic disorders and to elucidate the molecular mechanism.
Female obese, type 2 diabetic KK-A mice were fed diets supplemented with 0.3% or 0.8% GRO (w/w) for 4-12 weeks. Mice were euthanized and autopsied at 20 weeks old. The effects of GRO on lipid and glucose metabolism were evaluated by measuring physiological and biochemical markers using mRNA sequencing, quantitative reverse-transcription PCR, and western blot analyses.
Compared to mice fed the control diet, GRO-supplemented mice had reduced body and white adipose tissue weights, serum levels of triglycerides and cholesterol, and improved glucose tolerance, while food intake was not affected. We found remarkable reductions in the gene expression levels of stearoyl-coenzyme A desaturase 1 (Scd1) and pyruvate dehydrogenase kinase isoenzyme 4 (Pdk4) in the liver, in addition to decreased expression of fatty acid synthase (Fasn) in inguinal white adipose tissue (iWAT). These results suggest that GRO supplementation improves lipid profiles via reduced de novo lipogenesis in the liver and white adipose tissue. Glucose metabolism may also be improved by increased glycolysis in the liver.
Our analysis of long-term supplementation of GRO in obese and diabetic mice should provide novel insight into preventing insulin resistance and metabolic syndromes.
据报道，从甘草（Glycyrrhiza glabra Linne）根乙醇提取物中获得的富含黄酮类化合物的油（GRO）对健康有益。在本研究中，我们旨在确定长期给予GRO对代谢紊乱的影响，并阐明其分子机制。
将雌性肥胖2型糖尿病KK-A小鼠喂食补充有0.3%或0.8% GRO（w/w）的饮食4至12周。在20周龄时对小鼠实施安乐死并进行解剖。通过使用mRNA测序、定量逆转录PCR和蛋白质免疫印迹分析测量生理和生化标志物，评估GRO对脂质和葡萄糖代谢的影响。
与喂食对照饮食的小鼠相比，补充GRO的小鼠体重和白色脂肪组织重量减轻，血清甘油三酯和胆固醇水平降低，葡萄糖耐量改善，而食物摄入量不受影响。我们发现肝脏中硬脂酰辅酶A去饱和酶1（Scd1）和丙酮酸脱氢酶激酶同工酶4（Pdk4）的基因表达水平显著降低，此外腹股沟白色脂肪组织（iWAT）中脂肪酸合酶（Fasn）的表达也降低。这些结果表明，补充GRO可通过减少肝脏和白色脂肪组织中的从头脂肪生成来改善脂质谱。肝脏中糖酵解增加也可能改善葡萄糖代谢。
我们对肥胖和糖尿病小鼠长期补充GRO的分析应为预防胰岛素抵抗和代谢综合征提供新的见解。
