Wu Xu, Jiang Lin, Xu Faqiong, Cao Sumei, Chen Yuan, Zhang Yingyan, He Wenfeng, Yan Meijiao, Lian Senyang, Li Ang
College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, 350000, Fujian, People's Republic of China.
College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, 350000, Fujian, People's Republic of China.
Anim Reprod Sci. 2021 Jul;230:106762. doi: 10.1016/j.anireprosci.2021.106762. Epub 2021 May 8.
There are recent reports of the important functions of long noncoding RNAs (lncRNAs) in female reproductive and ovarian development. Studies in which there was characterization of lncRNAs in the ovaries of laying compared with nesting poultry, however, are limited. In this study, RNA libraries were constructed by obtaining sequencing data of ovarian tissues from laying and nesting Muscovy ducks. In the ovarian tissues of Muscovy ducks, a total of 334 differentially abundant mRNA transcripts (DEGs) and 36 differentially abundant lncRNA transcripts were identified in the nesting period, when compared with during the laying period. These results were subsequently validated by qRT-PCR using nine randomly-selected lncRNAs and six randomly-selected DAMTs. Furthermore, the cis- and trans-regulatory target genes of differentially abundant lncRNA transcripts were identified, and lncRNA-gene interaction networks of 34 differentially abundant lncRNAs and 263 DEGs were constructed. A total of 7601 lncRNAs neighboring 10,542 protein-coding genes were identified and found to be enriched in the Wnt signaling pathway and oocyte meiosis pathways associated with follicular development. Overall, only 11 cis-targets and 57 mRNA-mRNA except trans-targets were involved in the lncRNA-gene interaction networks. Based on the interaction networks, nine DEGs were trans-regulated by differentially abundant lncRNAs and 20 differentially abundant lncRNAs were hypothesized to have important functions in the regulation of broodiness in Muscovy ducks. In this study, a predicted interaction network of differentially abundant lncRNAs and DEGs in Muscovy ducks was constructed for the first time leading to an enhanced understanding of lncRNA and gene interactions regulating broodiness.
最近有报道称长链非编码RNA（lncRNAs）在雌性生殖和卵巢发育中具有重要功能。然而，与产蛋家禽相比，对抱窝家禽卵巢中lncRNAs进行特征描述的研究却很有限。在本研究中，通过获取产蛋和抱窝番鸭卵巢组织的测序数据构建了RNA文库。与产蛋期相比，在抱窝期的番鸭卵巢组织中，共鉴定出334个差异丰富的mRNA转录本（DEGs）和36个差异丰富的lncRNA转录本。随后，使用9个随机选择的lncRNAs和6个随机选择的DAMTs通过qRT-PCR对这些结果进行了验证。此外，还鉴定了差异丰富的lncRNA转录本的顺式和反式调控靶基因，并构建了34个差异丰富的lncRNAs和263个DEGs的lncRNA-基因相互作用网络。共鉴定出与10542个蛋白质编码基因相邻的7601个lncRNAs，并发现它们在与卵泡发育相关的Wnt信号通路和卵母细胞减数分裂通路中富集。总体而言，lncRNA-基因相互作用网络中仅涉及11个顺式靶标和57个除反式靶标外的mRNA-mRNA。基于相互作用网络，9个DEGs受到差异丰富的lncRNAs的反式调控，并且推测20个差异丰富的lncRNAs在番鸭抱窝调控中具有重要功能。在本研究中，首次构建了番鸭差异丰富的lncRNAs和DEGs的预测相互作用网络，从而增强了对lncRNA和调控抱窝的基因相互作用的理解。
