Department of Histology and Embryology, Medical University of Warsaw, Street Chałubińskiego 5, 02-004, Warsaw, Poland.
Department of Histology and Embryology, Medical University of Warsaw, Street Chałubińskiego 5, 02-004, Warsaw, Poland; Laboratory of Experimental Medicine, Medical University of Warsaw, Street Nielubowicza 5, 02-091, Warsaw, Poland; Department of Internal Medicine and Endocrinology, Medical University of Warsaw, Street Banacha 1A, 02-097, Warsaw, Poland.
Cancer Treat Res Commun. 2021;28:100385. doi: 10.1016/j.ctarc.2021.100385. Epub 2021 May 11.
Epithelial to mesenchymal transition (EMT) is a process associated with cancer malignancy and metastases. Cells undergoing EMT lose their epithelial phenotype and acquire mesenchymal phenotype. This process is accompanied by several molecular changes such as decrease of E-cadherin and increase of N-cadherin which is called the "cadherin swich". MicroRNAs (miRNAs, miRs) are small non-coding RNAs having ability to regulate genes post-transcriptionally. Nowadays they are believed to take part in multiple physiological and pathological processes including cancer development. Comparison between TargetScan7 (www.targetscan.org) results for miR-200b and metanalysis of genes involved in EMT showed that miR-200b has a potential binding site in 60 genes that are involved in EMT (the majority of them were associated with mesenchymal phenotype). Our review summarizes literature findings contributing to experimentally proven interactions between miR-200b and genes involved in EMT process including cell receptors, signaling pathways, cell cycle or cell adhesion. The results of those interactions indicate that miR-200b may have an inhibitory impact on EMT or even in selected cases is able to restore epithelial phenotype.
上皮-间充质转化（EMT）是与癌症恶性和转移相关的过程。经历 EMT 的细胞失去上皮表型并获得间充质表型。这个过程伴随着几个分子变化，如 E-钙黏蛋白的减少和 N-钙黏蛋白的增加，这被称为“钙黏蛋白开关”。微小 RNA（miRNAs，miRs）是具有转录后调节基因能力的小非编码 RNA。如今，它们被认为参与多种生理和病理过程，包括癌症的发展。miR-200b 的 TargetScan7（www.targetscan.org）结果比较和 EMT 相关基因的荟萃分析表明，miR-200b 在 60 个 EMT 相关基因中有一个潜在的结合位点（其中大多数与间充质表型有关）。我们的综述总结了文献中发现的实验证实的 miR-200b 与 EMT 过程中涉及的基因之间的相互作用，包括细胞受体、信号通路、细胞周期或细胞黏附。这些相互作用的结果表明，miR-200b 可能对 EMT 具有抑制作用，甚至在某些情况下能够恢复上皮表型。
