Birgens H S, Kristensen L O, Borregaard N, Karle H, Hansen N E
Department of Medicine and Haematology C, Gentofte Hospital, Hellerup, Denmark.
Eur J Haematol. 1988 Jul;41(1):52-7. doi: 10.1111/j.1600-0609.1988.tb00868.x.
Interactions of 125I-59Fe-lactoferrin with human monocytes were studied. After 4 hours of incubation, the uptake of 59Fe exceeded that of 125I. In dissociation studies the cellular 59Fe-activity was only partly dissociable during 16 h, whereas the 125I-activity could be released nearly completely. Disruption of the cells and studies on the cytosolic phase were performed employing gel-filtration and affinity chromatography. An appreciable amount of lactoferrin was found in the cytosolic phase. About 50% of the cytosolic 59Fe-activity was bound to ferritin. The results suggest that lactoferrin is internalized into monocytes and that iron is transferred to ferritin. These cellular events may contribute to an understanding of the accumulation of iron in the monocyte/macrophage system observed during inflammatory conditions.
研究了¹²⁵I - ⁵⁹Fe - 乳铁蛋白与人单核细胞的相互作用。孵育4小时后，⁵⁹Fe的摄取量超过了¹²⁵I。在解离研究中，细胞内的⁵⁹Fe活性在16小时内仅有部分可解离，而¹²⁵I活性几乎可完全释放。采用凝胶过滤和亲和层析对细胞进行破碎并研究胞质相。在胞质相中发现了相当数量的乳铁蛋白。约50%的胞质⁵⁹Fe活性与铁蛋白结合。结果表明乳铁蛋白被单核细胞内化，并且铁被转移到铁蛋白中。这些细胞事件可能有助于理解在炎症条件下观察到的单核细胞/巨噬细胞系统中铁的积累。
