Vogel M C, Papadopoulos T, Müller-Hermelink H K, Drahovsky D, Pfeifer G P
Center of Biological Chemistry, University of Frankfurt, FRG.
FEBS Lett. 1988 Aug 15;236(1):9-13. doi: 10.1016/0014-5793(88)80275-8.
The intracellular distribution of DNA methyltransferase has been analyzed in synchronously proliferating human cells. The localization of DNA methyltransferase was determined immunocytochemically using monoclonal antibodies directed against this enzyme. DNA methyltransferase was found to accumulate predominantly in nuclei with weak cytoplasmic staining. The DNA methyltransferase antigen was absent in early G1 phase, appeared in late G1 prior to the onset of DNA synthesis and persisted throughout S and G2 phases of the cell cycle. Mitotic cells showed a particularly strong staining intensity. These results show that DNA methyltransferase levels fluctuate during the cell cycle. This has possible implications on the stability of the DNA methylation pattern.
已对同步增殖的人类细胞中DNA甲基转移酶的细胞内分布进行了分析。使用针对该酶的单克隆抗体通过免疫细胞化学方法确定了DNA甲基转移酶的定位。发现DNA甲基转移酶主要在细胞核中积累，细胞质染色较弱。在细胞周期的G1期早期不存在DNA甲基转移酶抗原，在DNA合成开始前的G1期晚期出现，并在整个S期和G2期持续存在。有丝分裂细胞显示出特别强的染色强度。这些结果表明，DNA甲基转移酶水平在细胞周期中波动。这可能对DNA甲基化模式的稳定性有影响。
