Department of Comparative Biomedical Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS, USA.
Thad Cochran National Warmwater Aquaculture Center, Delta Research and Extension Center, Mississippi State University, Stoneville, MS, USA.
J Fish Dis. 2021 Sep;44(9):1399-1409. doi: 10.1111/jfd.13398. Epub 2021 May 24.
Blue catfish alloherpesvirus (BCAHV) is a novel virus isolated from the blue catfish (Ictalurus furcatus). To date, the ultrastructure, virulence and immunogenicity of BCAHV have not been reported. Given the importance of blue catfish in producing channel ♀ (I. punctatus) × ♂ blue (I. furcatus) catfish hybrids and the increasing demand for hybrid catfish in the US catfish industry, the susceptibility of blue, channel and hybrid catfish to BCAHV was assessed. Further, the cross-protective potential of BCAHV against Ictalurid herpesvirus 1 (IcHV1) was investigated in channel and hybrid catfish that survive BCAHV exposure. Neutralization assays revealed BCAHV is refractive (neutralization index [NI] = 0) to anti-IcHV1 monoclonal antibody Mab 95, compared to IcHV1 (NI = 1.8). Exposure of blue catfish fingerling to 1.3 × 10 TCID /L BCAHV produced cumulative mortality of 51.67 ± 0.70% and pathologic changes similar to those of channel catfish virus disease. No mortality was observed in channel or hybrid catfish. Twenty-eight days post-challenge, surviving channel and hybrid catfish were exposed to 9.4 × 10 TCID /L IcHV1 (LC dose), resulting in 100% relative per cent survival compared to naïve cohorts. These data provide baseline information for BCAHV and lay the groundwork for future studies. Data also identify BCAHV as a potential vaccine candidate against IcHV1. Based on host range and immunogenicity evaluations, in addition to genome sequence data from previous studies, BCAHV should be given consideration as a new species of Ictalurivirus.
蓝鲶虹彩病毒（BCAHV）是一种从蓝鲶（Ictalurus furcatus）中分离出来的新型病毒。迄今为止，尚未报道过 BCAHV 的超微结构、毒力和免疫原性。鉴于蓝鲶在生产斑点叉尾𫚔♀（Ictalurus punctatus）×♂蓝鲶（I. furcatus）杂交鲶中的重要性，以及美国鲶鱼产业对杂交鲶鱼需求的增加，评估了蓝鲶、斑点叉尾𫚔和杂交鲶对 BCAHV 的易感性。此外，还研究了在暴露于 BCAHV 后幸存的斑点叉尾𫚔和杂交鲶中，BCAHV 对 Ictalurid 疱疹病毒 1（IcHV1）的交叉保护潜力。中和试验表明，与 IcHV1（中和指数[NI] = 1.8）相比，BCAHV 对 IcHV1 单克隆抗体 Mab 95 具有折射性（NI = 0）。将蓝鲶鱼苗暴露于 1.3×10 TCID /L 的 BCAHV 中，累积死亡率为 51.67±0.70%，并产生与斑点叉尾𫚔病毒病相似的病理变化。在斑点叉尾𫚔或杂交鲶中未观察到死亡。攻毒后 28 天，幸存的斑点叉尾𫚔和杂交鲶鱼暴露于 9.4×10 TCID /L 的 IcHV1（LC 剂量）中，与未攻毒的对照组相比，相对存活率为 100%。这些数据为 BCAHV 提供了基线信息，并为未来的研究奠定了基础。数据还表明 BCAHV 是 IcHV1 的潜在疫苗候选物。基于宿主范围和免疫原性评估，以及之前研究的基因组序列数据，BCAHV 应被视为 Ictalurivirus 的一个新种。
