根分泌物中天然独脚金内酯的评价与定量。

Evaluation and Quantification of Natural Strigolactones from Root Exudates.

机构信息

Utsunomiya University, Utsunomiya, Japan.

Ehime University, Matsuyama, Japan.

出版信息

Methods Mol Biol. 2021;2309:3-12. doi: 10.1007/978-1-0716-1429-7_1.

DOI:10.1007/978-1-0716-1429-7_1

PMID:34028674

Abstract

Strigolactones (SLs) in the root exudates can be detected by germination assays with root parasitic weed seeds, but precise and accurate evaluation and quantification are possible only by chemical analysis with the liquid chromatography-tandem mass spectrometry (LC-MS/MS). Here we describe methods for root exudate collection, sample preparation, and LC-MS/MS analysis of SLs.

摘要

在根分泌物中可以通过与根寄生杂草种子的萌发试验来检测独脚金内酯 (SLs)，但只有通过液相色谱-串联质谱法 (LC-MS/MS) 的化学分析才能进行精确和准确的评估和定量。在这里，我们描述了根分泌物收集、样品制备以及 SLs 的 LC-MS/MS 分析方法。