Zhou Qian, Niu Changbin, Feng Liang, Dong Meixiu, Li Xiaoxu, Kong Bo, Li Changsheng
Hunan Research Center of the Basic Discipline for Cell Signaling, Hunan Provincial Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha, China.
Beijing Life Science Academy, Beijing, China.
Front Plant Sci. 2025 Jun 18;16:1618437. doi: 10.3389/fpls.2025.1618437. eCollection 2025.
Strigolactones are a newly identified group of phytohormones that regulate plant growth and development and also act as communication signals in the rhizosphere. Beyond their well-known activity in stimulating parasitic weed germination, strigolactones function in regulating plant architecture, promoting symbiosis with arbuscular mycorrhizal fungi, and modulating responses to various environmental stresses. However, their low abundance, structural diversity, and instability have hindered comprehensive research and their practices. In this review, from the perspective of biological researcher, we summarize the powerful tools and strategies related to chemistry and chemical biology used in strigolactone area, covering analytical chemistry tools for isolation and structural elucidation, synthetic chemistry for structural elucidation and agricultural applications, chemical biology and biosynthetic strategies for functional characterization. Biosensors and probes used in monitoring strigolactone activity and signaling were also highlighted. Finally, we address current challenges and discuss future research perspectives, aiming to provoke more investigations on strigolactone biology and further boost their agricultural practices.
独脚金内酯是一组新发现的植物激素，可调节植物的生长发育，还可作为根际中的通讯信号。除了在刺激寄生杂草萌发方面的众所周知的活性外，独脚金内酯还具有调节植物形态、促进与丛枝菌根真菌共生以及调节对各种环境胁迫的反应等功能。然而，它们的低丰度、结构多样性和不稳定性阻碍了全面的研究及其实际应用。在这篇综述中，我们从生物学研究者的角度，总结了独脚金内酯领域中与化学和化学生物学相关的强大工具和策略，涵盖用于分离和结构解析的分析化学工具、用于结构解析和农业应用的合成化学、用于功能表征的化学生物学和生物合成策略。还重点介绍了用于监测独脚金内酯活性和信号传导的生物传感器和探针。最后，我们阐述了当前的挑战并讨论了未来的研究前景，旨在激发更多关于独脚金内酯生物学的研究，并进一步推动其农业应用。
