Kramann Rafael, Menzel Sylvia
Institute of Experimental Medicine and Systems Biology, RWTH Aachen University Hospital, Aachen, Germany.
Department of Internal Medicine, Nephrology and Transplantation, Erasmus Medical Center, Rotterdam, The Netherlands.
Methods Mol Biol. 2021;2299:323-338. doi: 10.1007/978-1-0716-1382-5_22.
Chronic kidney disease (CKD) affects over 10% of the worldwide population and kidney fibrosis is a main driver of CKD and considered a therapeutic target. The mechanisms leading to kidney fibrosis are highly complexed and can be best studied in rodent models. Here we describe the most commonly used kidney fibrosis models in mice, the unilateral ureteral obstruction (UUO) model and the ischemia reperfusion injury (IRI) model. Both models are easy to learn and can be applied in animals of different age, sex, and strain.
慢性肾脏病（CKD）影响着全球超过10%的人口，肾纤维化是CKD的主要驱动因素，被视为一个治疗靶点。导致肾纤维化的机制高度复杂，在啮齿动物模型中研究最为合适。在此，我们描述小鼠中最常用的肾纤维化模型，即单侧输尿管梗阻（UUO）模型和缺血再灌注损伤（IRI）模型。这两种模型都易于操作，可应用于不同年龄、性别和品系的动物。
