Department of Plant Sciences, University of Cambridge, Cambridge, UK.
Methods Mol Biol. 2021;2317:343-365. doi: 10.1007/978-1-0716-1472-3_21.
The bryophyte Marchantia polymorpha , has attracted significant attention as a powerful experimental system for studying aspects of plant biology including synthetic biology applications. We describe an efficient and simple recursive Type IIS DNA assembly method for the generation of DNA constructs for chloroplast genome manipulation, and an optimized technique for Marchantia chloroplast genome transformation. The utility of the system was demonstrated by the expression of a chloroplast codon-optimized cyan fluorescent protein.
苔藓植物地钱作为一种强大的实验系统，已经引起了人们的广泛关注，可用于研究植物生物学的多个方面，包括合成生物学应用。我们描述了一种高效、简单的递归 II 型 DNA 组装方法，用于生成用于叶绿体基因组操作的 DNA 构建体，以及优化的地钱叶绿体基因组转化技术。该系统的实用性通过表达叶绿体密码子优化的青色荧光蛋白得到了验证。
