Occupational exposure to anaerobic bacteria in a waste sorting plant.

The study focused on exposure assessment to bacterial aerosols and organic dust in waste sorting plant. Samples were collected at different workplaces of waste sorting cycle i.e.: waste press, reloading area, loading of conveyor belt, sorting cabin, sorting hall, and control room. A quantitative analysis of aerobic and anaerobic bacteria was supplemented by qualitative analysis of anaerobic biota with the use of culture-based methods and biochemical tests. In addition, inhalable dust concentrations were also evaluated. To confirm the presence of genus, the PCR reaction with specific primers (Chis150f and ClostIr) was performed. The average concentration of total bacteria in waste sorting plant was 4347 CFU m (SD = 2439), of which 66% were anaerobic strains (2852 CFU m; SD = 2127). It was found that about 24% of anaerobic bacteria belonged to genus (682 CFU m; SD = 633). The highest contamination with anaerobic bacteria was observed near the waste reloading plant (3740 CFU m), and the lowest in the control room (850 CFU m). The average concentration of inhalable dust in the waste sorting plant was 0.81 mg m (SD = 0.59). The correlation analysis showed that the presence of anaerobic bacteria, including clostridia was significantly determined by the microclimate parameters. Qualitative analysis showed the presence of 16 anaerobic species belonging to 9 genera, of which , and were present at all workplaces. The molecular analysis confirmed the presence of genus in both bioaerosol and settled dust samples.: The study showed that anaerobic bacteria should be taken into account as an important component of this microbiota when assessing the exposure of waste sorting workers to biological agents. However, future studies should investigate more precisely how the composition of sorted waste as well as the season can affect the diversity of anaerobic bacteria in this working environment. More attention should be paid to regular cleaning of equipment surfaces in the plant, as deposited organic dust is an important reservoir of anaerobic bacteria, including those of a potentially pathogenic nature.