Bodrova Valeria V, Shustova Olga N, Khaspekova Svetalana G, Mazurov Alexey V
National Medical Research Center for Cardiology, Russian Ministry of Health, Moscow, Russian Federation.
Platelets. 2022 Apr 3;33(3):398-403. doi: 10.1080/09537104.2021.1922659. Epub 2021 May 24.
Reticulated platelets (RP) are young, functionally active platelet forms which are detected by RNA staining. Their content in the circulation reflects the intensity of bone marrow thrombocytopoesis. The aim of this study was to assess in healthy volunteers the relationship between RP percentage and platelet size and activity. RP were quantitated by thiazole orange staining using flow cytometry. Platelet size indexes included mean platelet volume (MPV), platelet large cell ratio (-LCR) measured in a Coulter type hematological analyzer and forward scattering (FSC) measured in a flow cytometer. Platelet functional activity was evaluated by expression of activated glycoprotein (GP) IIb-IIIa (PAC-1 antibody binding) and -selectin with the use of flow cytometry. Platelets were activated by thrombin receptor activating peptide (TRAP) (10 and 1 µM) and ADP (20 and 2.5 µM). The percentage of RP in healthy volunteers varied from 2.9% to 23.8% (mean ± SD ‒ 11.7 ± 4.7%, n = 99) and correlated with all platelet size indexes: MPV, -LCR and FCS (r from 0.452 to 0.529, < .001, n = 87-99). On average, RP were distributed at a ratio of 9:1 between 50% subpopulations of large and small platelets according to their FSC index. Expression of GP IIb-IIIa activated form correlated with RP percentage and platelet size indexes when platelets were activated by TRAP and ADP at both applied concentrations (r from 0.309 to 0.560, p from 0.014 to < 0.001, n = 50-62). -selectin expression correlated with RP percentage and platelet size indexes when platelets were activated by 10 µM TRAP inducing maximum expression of this activation marker (r from 0.332 to 0.556, p from 0.008 to < 0.001, n = 65), but not by weaker agonists: 1 µM TRAP, 20 and 2.5 µM ADP (r < 0.3, n = 54-66). Thus, high RP content in healthy volunteers is associated with increased platelet size and activity in the whole platelet population.
网织血小板（RP）是年轻的、功能活跃的血小板形式，可通过RNA染色检测到。它们在循环中的含量反映了骨髓血小板生成的强度。本研究的目的是评估健康志愿者中网织血小板百分比与血小板大小和活性之间的关系。通过流式细胞术使用噻唑橙染色对网织血小板进行定量。血小板大小指标包括在库尔特型血液分析仪中测量的平均血小板体积（MPV）、血小板大细胞比率（-LCR）以及在流式细胞仪中测量的前向散射（FSC）。通过使用流式细胞术检测活化糖蛋白（GP）IIb-IIIa（PAC-1抗体结合）和P-选择素的表达来评估血小板功能活性。血小板用凝血酶受体激活肽（TRAP）（10和1μM）和ADP（20和2.5μM）激活。健康志愿者中网织血小板的百分比在2.9%至23.8%之间变化（平均值±标准差‒11.7±4.7%，n = 99），并且与所有血小板大小指标相关：MPV、-LCR和FCS（r为0.452至0.529，p <.001，n = 87 - 99）。根据FSC指标，平均而言，网织血小板在大小血小板50%亚群之间的分布比例为9:1。当血小板在两种应用浓度下被TRAP和ADP激活时，活化形式的GP IIb-IIIa的表达与网织血小板百分比和血小板大小指标相关（r为0.309至0.560，p为0.014至< 0.001，n = 50 - 62）。当血小板被诱导该激活标志物最大表达的10μM TRAP激活时，P-选择素表达与网织血小板百分比和血小板大小指标相关（r为0.332至0.556，p为0.008至< 0.001，n = 65），但与较弱的激动剂：1μM TRAP、20和2.5μM ADP无关（r < 0.3，n = 54 - 66）。因此，健康志愿者中网织血小板含量高与整个血小板群体中血小板大小和活性增加有关。
