Department of Biology, Trent University, Peterborough, Ontario, K9L 0G2, Canada.
Department of Biology, Trent University, Peterborough, Ontario, K9L 0G2, Canada.
J Biotechnol. 2021 Jun 20;334:43-46. doi: 10.1016/j.jbiotec.2021.05.005. Epub 2021 May 21.
The number of organisms that are known to produce cytokinins (CKs) continues to increase. In fact, species from all life kingdoms have now been shown to either produce CKs or at least have the genetic components to make it possible. In vitro growth of microorganisms, plant/animal cells, and tissue cultures often requires nutrient-rich media composed of ingredients with organic origins including: yeast extract, peptone, tryptone, or various plant or animal extracts. These compounds, derived from microbial, plant and animal materials, can be the source of significant levels of exogenous CKs in the culture medium. As CK investigative work continues to expand rapidly, it is of critical importance to draw attention to this complexity; the presence of CKs in growth medium affects CK metabolism of the cultured organism and interferes with the readings of analytical instrumentation used to profile CKs in tested microorganisms or cell cultures.
已知产生细胞分裂素 (CKs) 的生物数量持续增加。事实上，现在已经证明所有生命王国的物种要么产生 CKs，要么至少具有使这成为可能的遗传成分。微生物、植物/动物细胞和组织培养的体外生长通常需要营养丰富的培养基，这些培养基由具有有机来源的成分组成，包括：酵母提取物、蛋白胨、胰蛋白胨或各种植物或动物提取物。这些源自微生物、植物和动物材料的化合物可以成为培养基中高水平外源 CKs 的来源。随着 CK 研究工作的快速扩展，引起对这种复杂性的关注至关重要；生长培养基中 CK 的存在会影响培养物的 CK 代谢，并干扰用于分析测试微生物或细胞培养物中 CK 的分析仪器的读数。
