高通量 miRNA 测序的人类胎盘: 整个孕期的表达。
High-throughput miRNA sequencing of the human placenta: expression throughout gestation.
机构信息
Department of Obstetrics & Gynecology, Center for Reproductive Medicine, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
出版信息
Epigenomics. 2021 Jul;13(13):995-1012. doi: 10.2217/epi-2021-0055. Epub 2021 May 25.
To understand miRNA changes across gestation in healthy human placentae. This is essential before miRNAs can be used as biomarkers or prognostic indicators during pregnancy. Using next-generation sequencing, we characterize the normative human placenta miRNome in first (n = 113) and third trimester (n = 47). There are 801 miRNAs expressed in both first and third trimester, including 182 with similar expression across gestation (p ≥ 0.05, fold change ≤2) and 180 significantly different (false discovery rate <0.05, fold change >2). Of placenta-specific miRNA clusters, chromosome 14 miRNA cluster decreases across gestation and chromosome 19 miRNA cluster is overall highly expressed. Chromosome 13 clusters are upregulated in first trimester. This work provides a rich atlas of healthy pregnancies to direct functional studies investigating the epigenetic differences in first and third trimester placentae.
为了了解健康人胎盘在妊娠过程中的 miRNA 变化。这在 miRNA 可以作为妊娠期间的生物标志物或预后指标之前是必不可少的。使用下一代测序，我们对第一个（n = 113）和第三个（n = 47） trimester 进行了规范化的人类胎盘 miRNome 特征描述。有 801 个 miRNA 在第一和第三个 trimester 都有表达，其中 182 个在整个妊娠过程中表达相似（p≥0.05，倍数变化≤2），180 个则显著不同（假发现率<0.05，倍数变化>2）。胎盘特异性 miRNA 簇中，染色体 14 miRNA 簇随妊娠而减少，染色体 19 miRNA 簇总体上高度表达。染色体 13 簇在第一个 trimester 上调。这项工作提供了一个丰富的健康妊娠图谱，以指导功能研究，调查第一和第三个 trimester 胎盘的表观遗传差异。
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