Division of Comparative Pathology and Department of Microbiology and Immunology, Tulane National Primate Research Center, Tulane University, Covington, LA, USA.
Methods Mol Biol. 2021;2311:39-49. doi: 10.1007/978-1-0716-1437-2_4.
This chapter describes the culture and propagation of murine embryonic stem cells, F9 and P19, and strategies for differentiation of these stem cells into neurons. Additional techniques are described for obtaining enriched populations of mature neurons from P19 cells and differentiation of F9 cells into serotonergic or catecholaminergic neurons. The protocols described herein can be used for dissection of the pathways such as gliogenesis and neurogenesis that are involved in differentiation of pluripotent stem cells such as F9 and P19 into glial cells or terminally differentiated neurons.
本章描述了小鼠胚胎干细胞（ES 细胞）F9 和 P19 的培养和传代，以及将这些干细胞分化为神经元的策略。还介绍了从 P19 细胞中获得成熟神经元的富集群体和将 F9 细胞分化为 5-羟色胺能或儿茶酚胺能神经元的其他技术。本文所述的方案可用于研究诸如神经发生和胶质发生等途径，这些途径参与了 F9 和 P19 等多能干细胞分化为神经胶质细胞或终末分化神经元。
