Shriners Hospital Pediatric Research Center, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA.
Methods Mol Biol. 2021;2311:167-175. doi: 10.1007/978-1-0716-1437-2_13.
The use of sensory neurons and assessment of neurite outgrowth in vitro is an important part of understanding neuronal development and plasticity. Cultures of rat dorsal root ganglion (DRG) neurons provide quantitative results very quickly and, when grown on growth promoting or inhibitory substrates, can be utilized to study axonal growth, neurotrophic dependence, and structure and function of growth cones. Since we are interested in axon regeneration and targeting, we have sought to promote neurite outgrowth by refining the techniques of growing DRG neurons in culture. This chapter describes detailed methods for the dissection and purification of DRG neurons and quantitative assessment of neurite on promoting or inhibitory substrates.
在体外使用感觉神经元并评估神经突生长是理解神经元发育和可塑性的重要部分。培养大鼠背根神经节 (DRG) 神经元可以快速获得定量结果，并且当在促进或抑制轴突生长的基质上生长时，可用于研究轴突生长、神经营养因子依赖性以及生长锥的结构和功能。由于我们对轴突再生和靶向感兴趣，因此我们一直在寻求通过改进 DRG 神经元在培养中的生长技术来促进神经突生长。本章描述了详细的 DRG 神经元解剖和纯化方法，以及在促进或抑制轴突生长的基质上定量评估神经突的方法。
