Division of Pediatric Oncology, Sidney Kimmel Cancer Center, Johns Hopkins University, Baltimore, MD, USA.
United States Navy, Philadelphia, PA, USA.
Methods Mol Biol. 2021;2255:159-169. doi: 10.1007/978-1-0716-1162-3_14.
Cytotoxic T cell-induced cell death is well documented. Cytotoxic T cell releases various cytolytic proteins. The cytolytic proteins induce target cell death. T cell-induced cell death can be measured by the lytic assay. One of the well-known lytic assays uses radioactive tracer, Chromium-51 (Cr), and detects the amount of Cr released from target cells. This assay can detect cell death and the efficiency of the T cell-induced cell death by coculture effector cells (T cells) and target cells. This assay can determine the kinetics of the cell lysis. The issue of this approach is the use of radioactive material. This chapter describes measuring T cell-induced cell death by determining the epigenetic remodeling and the release of cytolytic proteins. Determine the efficiency of T cell-induced cell death by using a flow cytometry-based detection method.
细胞毒性 T 细胞诱导的细胞死亡已有充分的文献记载。细胞毒性 T 细胞释放各种细胞溶素蛋白。这些细胞溶素蛋白诱导靶细胞死亡。T 细胞诱导的细胞死亡可以通过细胞溶解测定来测量。众所周知的细胞溶解测定之一使用放射性示踪剂铬-51（Cr），并检测从靶细胞释放的 Cr 的量。该测定法可通过共培养效应细胞（T 细胞）和靶细胞来检测细胞死亡和 T 细胞诱导的细胞死亡的效率。该测定法可确定细胞裂解的动力学。该方法的问题是使用放射性物质。本章描述了通过确定表观遗传重塑和细胞溶素蛋白的释放来测量 T 细胞诱导的细胞死亡。通过基于流式细胞术的检测方法确定 T 细胞诱导的细胞死亡的效率。
