South West Acute Hospital - Paediatric Department, Northern Ireland, 124 Irvinestown Rd, Enniskillen, BT 74 6DN, United Kingdom.
J Virol Methods. 2021 Aug;294:114179. doi: 10.1016/j.jviromet.2021.114179. Epub 2021 May 24.
Rotavirus A (RV) is the primary cause of gastroenteritis in children worldwide and a leading cause of gastroenteritis in children younger than three years, with a significant burden both globally and in Ireland. Rotavirus vaccine (Rotarix™) was introduced into Ireland in 2016. The aim of this study was to determine the diversity and frequency of, and predominant, RV genotypes, nosocomial acquisition, viral co-infections and severity of RV infection in Ireland in the post-vaccination year, from November 18th 2016 to November 18th 2017. The study included all children up to 3 years of age who had presented to Mayo University Hospital or were admitted with vomiting and diarrhoea, and had their stool tested for rotavirus and other viruses by real-time PCR in the National Virus Reference Laboratory. The Vesikari Scoring System was used to assess disease severity. The results showed that rotavirus was a leading cause of gastroenteritis (37 patients, 24.6 % of a total of 150 patients) and gastroenteritis-related hospitalisation (27 patients were admitted, 21 % of a total of 128 patients). Severe rotaviral gastroenteritis was noted in 78 % of all RV gastroenteritis (37 patients). The RV strain G1P[8], including the vaccine G1P[8] strain (Rotarix™), was the most predominant genotype (47 %), followed by G2P[4] (31 %), G4P[8] (8%), G12P[8] (8%) and G9P[8] (6%). RV co-infection with other viruses was detected in four cases (11 %), of whom three cases (75 %) were severe. Rotarix™ was detected in six vaccinated patients (35 %), 50 % were mild disease. Nosocomial infection was detected in one case. These results indicated that RV remained the leading cause of paediatric gastroenteritis during the post vaccination year in Ireland.
轮状病毒 A（RV）是全球儿童中胃肠炎的主要原因，也是 3 岁以下儿童胃肠炎的主要原因，在全球和爱尔兰都有很大的负担。轮状病毒疫苗（Rotarix™）于 2016 年在爱尔兰推出。本研究的目的是确定轮状病毒疫苗接种后一年（2016 年 11 月 18 日至 2017 年 11 月 18 日）在爱尔兰，病毒的多样性和频率、主要基因型、医院获得性感染、病毒合并感染和轮状病毒感染的严重程度。该研究包括所有 3 岁以下因呕吐和腹泻到梅奥大学医院就诊或住院的儿童，他们的粪便在国家病毒参考实验室通过实时 PCR 检测轮状病毒和其他病毒。Vesikari 评分系统用于评估疾病严重程度。结果表明，轮状病毒是导致胃肠炎（150 例患者中有 37 例，占 24.6%）和胃肠炎相关住院（128 例患者中有 27 例住院，占 21%）的主要原因。所有轮状病毒胃肠炎中，严重轮状病毒性胃肠炎占 78%（37 例）。RV 株 G1P[8]，包括疫苗 G1P[8]株（Rotarix™），是最主要的基因型（47%），其次是 G2P[4]（31%）、G4P[8]（8%）、G12P[8]（8%）和 G9P[8]（6%）。在 4 例（11%）中检测到轮状病毒与其他病毒的合并感染，其中 3 例（75%）为严重感染。在 6 名接种疫苗的患者（35%）中检测到 Rotarix™，其中 50%为轻度疾病。检测到 1 例医院获得性感染。这些结果表明，轮状病毒在爱尔兰疫苗接种后一年仍是导致儿科胃肠炎的主要原因。
